Protein Info for PFLU_RS15745 in Pseudomonas fluorescens SBW25-INTG

Annotation: type II secretion system ATPase GspE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 TIGR02533: type II secretion system protein E" amino acids 2 to 469 (468 residues), 737.8 bits, see alignment E=2.6e-226 PF00437: T2SSE" amino acids 111 to 377 (267 residues), 349.4 bits, see alignment E=5.7e-109

Best Hits

Swiss-Prot: 86% identical to HXCR_PSEAE: Probable type II secretion system protein HxcR (hxcR) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02454, general secretion pathway protein E (inferred from 100% identity to pfs:PFLU3231)

Predicted SEED Role

"General secretion pathway protein E"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KC61 at UniProt or InterPro

Protein Sequence (472 amino acids)

>PFLU_RS15745 type II secretion system ATPase GspE (Pseudomonas fluorescens SBW25-INTG)
MNALPYAWAKAQRILLRNGEEGAVLTVCPSTPGWSISEVRRQFGAARLEQVRDEELDGLL
AAAYADTGSAAAVVGAAENEVDLDRLMQDIPEITDLLDTQDGAPVIRMINALLTQAARDE
ASDIHIEPYESHSVVRYRVDGTLRDVVSPRKALHAALVSRIKIMAQLDIAEKRLPQDGRI
ALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLQLETLGMDVQVLAKLDNLIRQPHG
IVLVTGPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFALA
LRAILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPF
LLASSMLGVLAQRLVRRLCSHCKQEDPAHPGTWRPLGCAACNHTGYSGRTGIHELFCIDD
EIRALIHQGAGEQALRLAAREAGMFSLREDGERWVRAGATAPEEILRVTRDA