Protein Info for PFLU_RS15590 in Pseudomonas fluorescens SBW25-INTG

Annotation: beta-ketoacyl-[acyl-carrier-protein] synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00109: ketoacyl-synt
  • amino acids 4 to 243 (240 residues), 162.6 bits, E=1.4e-51
• PF02801: Ketoacyl-synt_C
  • amino acids 253 to 363 (111 residues), 120.4 bits, E=4.5e-39

Best Hits

Swiss-Prot: 47% identical to FABF_ECOL6: 3-oxoacyl-[acyl-carrier-protein] synthase 2 (fabF) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K09458, 3-oxoacyl-[acyl-carrier-protein] synthase II [EC: 2.3.1.179] (inferred from 100% identity to pfs:PFLU3199)

MetaCyc: 47% identical to 3-oxoacyl-[acyl carrier protein] synthase 2 (Escherichia coli K-12 substr. MG1655)
Beta-ketoacyl-acyl-carrier-protein synthase II. [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179,2.3.1.41 [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179,2.3.1.41 [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179 [EC: 2.3.1.179, 2.3.1.41]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• biotin biosynthesis I (13/15 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• (5Z)-dodecenoate biosynthesis II (6/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• fatty acid elongation -- saturated (4/5 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• stearate biosynthesis IV (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• petroselinate biosynthesis (2/6 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (20/205 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.179, 2.3.1.41

Use Curated BLAST to search for 2.3.1.179 or 2.3.1.41

Sequence Analysis Tools

See C3KC29 at UniProt or InterPro

Protein Sequence (405 amino acids)

>PFLU_RS15590 beta-ketoacyl-[acyl-carrier-protein] synthase family protein (Pseudomonas fluorescens SBW25-INTG)
MVAEAVVVTGIGLVTPAGIGIEANWRRVVAGEATATPDKTLKAMPVEISCRVPGFDSALL
GVKSPWQWDRYAQFALLAARQALEDAGLKAGSWKDESRVAVIIGSGAGGTATLEQQHAVL
IAEGPDDVSSLTLPMGLLNMAAGQVAIELGARGPCLAPCSACASGASALGMAKDLINRGL
CDIVVAGGAEAPITPLYVSAFAKMRALSRNTDAGGASRPFDVARDGFVIGEGAGMFVLES
EAHARARGARSHGRLLGYGASADAHHVTTPHPQGLGARLAMQAALRDAGIEADAVDYVNA
HGTSTPGNDVIESRAIAELFGARMPVSSTKGVTGHLLGAAGAIEAAYTLLALEHGLLPPT
ANLRNPDPQITVDLVHGQARAQPIKVALSNSFGFGGQNVSLVFAA