Protein Info for PFLU_RS15555 in Pseudomonas fluorescens SBW25-INTG

Annotation: ShlB/FhaC/HecB family hemolysin secretion/activation protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 573 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF08479: POTRA_2" amino acids 80 to 155 (76 residues), 81.5 bits, see alignment E=4.8e-27 PF17287: POTRA_3" amino acids 157 to 211 (55 residues), 73.3 bits, see alignment 1.3e-24 PF03865: ShlB" amino acids 216 to 533 (318 residues), 318.8 bits, see alignment E=7.1e-99

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3192)

Predicted SEED Role

"Channel-forming transporter/cytolysins activator of TpsB family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KC22 at UniProt or InterPro

Protein Sequence (573 amino acids)

>PFLU_RS15555 ShlB/FhaC/HecB family hemolysin secretion/activation protein (Pseudomonas fluorescens SBW25-INTG)
MWRSVRRTRLCLLFAFFCLSTAGMAQGATPQDTPGVTDLIRDRQDRLLEEQQRRLEELKD
LPGKAAAPAKPAAPADTRCFAINTIELSGADALSEGERASLIKPYIGQCLGVPQLNDLLK
VITDHYLAKGLVTSRAYLPQQNLGSGNLKVQVVEGRLEGMKGAEGSGITDRQLAMSFPGK
PGDLLNLREIEQMVDQLNRLPSNQAQMELAPGKAVGGSEVLVKNTPQKPWRVGLSRHNGG
QRSTGEQQWGASLDWDNPLGLADQLSLRGGHDAISDHQKTSRNSMLNYNLPFGWWNLNYT
YSESDYRSLAQASGFNFKQSGDSQNHQLRLERVIHRDALSKTSLSTGVAYLRTNNFIEDS
KLALSSNRLSEAQFGINHGRRIGGSFLNIDLGMQDGIGAFDAQANHNPRPGQADARYRKY
TATVSYLYPFKAWGESFSFSSLMTGQRSEDVLFSPQRMSLGGQSSIRGYKDQSLSGYSGG
YWRNDLRWSRPVTFAWLQPVFAEYGTSLGYDQGVIRRDRYNAEQHGRMTSNSVDLFARGQ
NVAATVTFAHSLERPGPITEREAPIYFRLDFFL