Protein Info for PFLU_RS15395 in Pseudomonas fluorescens SBW25-INTG

Annotation: alpha-D-glucose phosphate-specific phosphoglucomutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01132: phosphoglucomutase, alpha-D-glucose phosphate-specific
  • amino acids 1 to 545 (545 residues), 875 bits, E=8.7e-268
• PF02878: PGM_PMM_I
  • amino acids 40 to 180 (141 residues), 110.5 bits, E=1.1e-35
• PF02879: PGM_PMM_II
  • amino acids 211 to 318 (108 residues), 50.7 bits, E=4.6e-17
• PF02880: PGM_PMM_III
  • amino acids 323 to 438 (116 residues), 97.2 bits, E=1.4e-31
• PF00408: PGM_PMM_IV
  • amino acids 493 to 536 (44 residues), 28.7 bits, 2.2e-10

Best Hits

Swiss-Prot: 64% identical to PGM_KOMXY: Phosphoglucomutase (celB) from Komagataeibacter xylinus

KEGG orthology group: K01835, phosphoglucomutase [EC: 5.4.2.2] (inferred from 100% identity to pfs:PFLU3157)

MetaCyc: 60% identical to phosphoglucomutase (Yersinia enterocolitica (type O:8))
Phosphoglucomutase. [EC: 5.4.2.2]

Predicted SEED Role

"Phosphoglucomutase (EC 5.4.2.2)" (EC 5.4.2.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• glycogen degradation II (5/6 steps found)
• UDP-α-D-glucose biosynthesis (2/2 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• glucose and glucose-1-phosphate degradation (4/5 steps found)
• sucrose degradation II (sucrose synthase) (4/5 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycogen degradation I (6/8 steps found)
• sucrose biosynthesis II (6/8 steps found)
• glycogen biosynthesis I (from ADP-D-Glucose) (3/4 steps found)
• starch degradation V (3/4 steps found)
• sucrose degradation IV (sucrose phosphorylase) (3/4 steps found)
• GDP-α-D-glucose biosynthesis (2/3 steps found)
• trehalose degradation V (2/3 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• starch degradation III (2/4 steps found)
• chitin biosynthesis (5/9 steps found)
• glucosylglycerol biosynthesis (2/5 steps found)
• starch biosynthesis (5/10 steps found)
• CDP-6-deoxy-D-gulose biosynthesis (1/5 steps found)
• glycogen biosynthesis III (from α-maltose 1-phosphate) (3/8 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• streptomycin biosynthesis (2/18 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Pentose phosphate pathway
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.4.2.2

Use Curated BLAST to search for 5.4.2.2

Sequence Analysis Tools

See C3KBP9 at UniProt or InterPro

Protein Sequence (548 amino acids)

>PFLU_RS15395 alpha-D-glucose phosphate-specific phosphoglucomutase (Pseudomonas fluorescens SBW25-INTG)
MTISPFAGKPAPAQLLVDIPRLVTAYYTGQPDAAISTQRVAFGTSGHRGSSFELSFNEWH
VLAISQAICLYREAQGINGPLFVGLDTHALSTPAGASALEVLAANGVHVMLAEGDEYTPT
PAISHAIICYNRGRTSGLADGIVITPSHNPPQSGGYKYNPPNGGPADTHVTKWIEAKANE
LLANKLAGVKRMTHAQALKADTTHRHDYLNSYVADLVNVIDMDAIRSADLRLGVDPLGGA
GVRYWSAIAEHYRLNLEVVNTEVDATFRFMSVDWDGQIRMDPSSSYAMQGLIGLKERFDV
AFACDPDHDRHGIVTPSGGLLAPNNYLAVSIDYLFQNRPDWRADAAVGKTVVSSGLIDRV
AARIGRRLYEVPVGFKWFADGLFEGSLGFGGEESAGASFLRKDGTVWSTDKDGLIPALLA
AEMTSRKGQDPSQIYRGLTDALGEPFAIRVDAKATPAQKALLGKLSPDQVTSTQLAGESI
QQILSHAPGNNQAIGGLKVMTENGWFAARPSGTEDIYKIYAESFIGEDHLKQLVEEAQVL
VDGAISAN