Protein Info for PFLU_RS15325 in Pseudomonas fluorescens SBW25-INTG

Annotation: HlyD family type I secretion periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 transmembrane" amino acids 21 to 39 (19 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 18 to 437 (420 residues), 385.2 bits, see alignment E=2.1e-119 PF00529: CusB_dom_1" amino acids 30 to 392 (363 residues), 52.6 bits, see alignment E=9e-18 PF13533: Biotin_lipoyl_2" amino acids 61 to 104 (44 residues), 36.9 bits, see alignment 6.2e-13 PF13437: HlyD_3" amino acids 289 to 388 (100 residues), 53.3 bits, see alignment E=1.1e-17

Best Hits

Swiss-Prot: 50% identical to APRE_PSEAE: Alkaline protease secretion protein AprE (aprE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12537, protease secretion protein HasE (inferred from 100% identity to pfs:PFLU3143)

Predicted SEED Role

"ABC-type protease exporter, membrane fusion protein (MFP) family component PrtE/AprE" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBF1 at UniProt or InterPro

Protein Sequence (437 amino acids)

>PFLU_RS15325 HlyD family type I secretion periplasmic adaptor subunit (Pseudomonas fluorescens SBW25-INTG)
MSSITVEPRFKERDATFFTRMGWLLTLVGAGGFFLWASLAPLDQGIPVQGTVVVSGKRKA
VQTLSPGVVSRILVKEGEHVKQGQPLFRLDQTQHQADVQSLQAQYRMAWASVARWQSERD
NHASIRFPAELSANPDPALALVLEGQRQLFSSRREAFAREQAGIRANIDGASAQLGGMRR
ARSDLTAQAQSLRDQLSNLQPLADNGYIPRNRLMEYQRQLSQVQQDLAQNSGESGRVEQG
ILESRLKLQQHSEEYQKEVRSQLADAQLRSLTLEQQLTSAGFDLQHSEINAPADGIAVNL
SVHTEGAVVRAGETLLEIVPQGTRLEVEGHLPVHLVDKVGTHLPVDILFTAFNQSRTPRV
PGEVSLISADQMLDEKTGAPYYVLRTTVSDAALEKLHGLVIKPGMPAEMFVRTGERSLLN
YLFKPLLDRAGSALTEE