Protein Info for PFLU_RS14785 in Pseudomonas fluorescens SBW25-INTG

Annotation: enoyl-CoA hydratase/isomerase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 24 to 196 (173 residues), 113.6 bits, E=1e-36
• PF16113: ECH_2
  • amino acids 27 to 359 (333 residues), 413.3 bits, E=9.7e-128

Best Hits

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to pfs:PFLU3032)

Predicted SEED Role

"3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 3.1.2.4)

MetaCyc Pathways

• oleate β-oxidation (33/35 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• adipate degradation (5/5 steps found)
• fatty acid β-oxidation I (generic) (6/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (4/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (4/5 steps found)
• propanoyl-CoA degradation II (4/5 steps found)
• L-valine degradation I (6/8 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (5/7 steps found)
• methyl ketone biosynthesis (engineered) (4/6 steps found)
• valproate β-oxidation (6/9 steps found)
• acrylate degradation I (3/5 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• phenylacetate degradation I (aerobic) (4/9 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (3/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (3/14 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 3.1.2.4 or 4.2.1.17

Sequence Analysis Tools

See C3KAC3 at UniProt or InterPro

Protein Sequence (367 amino acids)

>PFLU_RS14785 enoyl-CoA hydratase/isomerase family protein (Pseudomonas fluorescens SBW25-INTG)
MTAQVSTEASHAAILQDEVLAEVRNHIGHLTLNRPAGLNALTLQMVRSLTSQLQAWSDDP
QVYAVVLRGAGEKAFCAGGDIRSLYDSFKNGDTLHQDFFVEEYALDLAIHHYRKPVLALM
DGFVLGGGMGLVQGADLRVVTERSRLAMPEVAIGYFPDVGGSYFLPRIPGELGIYLGVTG
VQIRAADALYCGLADWYLDSHKLADLDQKLDNLRWHDSPLKDLQGALARLAVQQLPDAPL
AALRPAIDHFFALPDVPSIVEQLQQVTVADSHEWALNTVSLMQTRSPLAMAVTLEMLRRG
RRLSLEQCFALELHLDRQWFERGDLIEGVRALIIDKDKSPRWNPPTLHGLALNHVESFFH
HFEKVVK