Protein Info for PFLU_RS14720 in Pseudomonas fluorescens SBW25-INTG
Annotation: chemotaxis protein CheB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 44% identical to CHEB2_LEPIC: Putative protein-glutamate methylesterase/protein-glutamine glutaminase (cheB2) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
KEGG orthology group: K03412, two-component system, chemotaxis family, response regulator CheB [EC: 3.1.1.61] (inferred from 100% identity to pfs:PFLU3019)Predicted SEED Role
"Protein-glutamate methylesterase (EC 3.1.1.61)" (EC 3.1.1.61)
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.61
Use Curated BLAST to search for 3.1.1.61
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3KAB0 at UniProt or InterPro
Protein Sequence (201 amino acids)
>PFLU_RS14720 chemotaxis protein CheB (Pseudomonas fluorescens SBW25-INTG) MNQAAASPAKVRGIEAIVIGASAGGVEALLIVFGQLPADFGLPIVAVLHLPDERRSQLAE VFDRRVAIPVVEARDKETLKPGTLYFAGPGYHLSVEQDRSLSLSQEDRVHHSRPAIDFLF ESAAEAYGKGLMAILLTGANHDGARGLAYVKRQGGTTVVQDPTEARVDVMPRAALALHTP DHILTLSRIGSLLASLEYSPC