Protein Info for PFLU_RS14645 in Pseudomonas fluorescens SBW25-INTG

Annotation: glycerate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00045: glycerate kinase
  • amino acids 2 to 375 (374 residues), 510 bits, E=2e-157
• PF02595: Gly_kinase
  • amino acids 3 to 373 (371 residues), 525.5 bits, E=3.6e-162

Best Hits

Swiss-Prot: 52% identical to GLXK_BACSU: Glycerate kinase (glxK) from Bacillus subtilis (strain 168)

KEGG orthology group: K00865, glycerate kinase [EC: 2.7.1.31] (inferred from 100% identity to pfs:PFLU3004)

MetaCyc: 91% identical to glycerate 2-kinase (Pseudomonas simiae)
GKI-RXN [EC: 2.7.1.165]

Predicted SEED Role

"Glycerate kinase (EC 2.7.1.31)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Glycine and Serine Utilization or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle (EC 2.7.1.31)

MetaCyc Pathways

• 2-deoxy-D-ribose degradation II (7/8 steps found)
• superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
• D-glucarate degradation I (3/4 steps found)
• D-galactarate degradation I (3/4 steps found)
• glycolate and glyoxylate degradation I (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• 2-O-α-mannosyl-D-glycerate degradation (1/2 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
• photorespiration II (6/10 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.31

Use Curated BLAST to search for 2.7.1.165 or 2.7.1.31

Sequence Analysis Tools

See C3KA95 at UniProt or InterPro

Protein Sequence (380 amino acids)

>PFLU_RS14645 glycerate kinase (Pseudomonas fluorescens SBW25-INTG)
MKIIIAPDSFKDSLSAEGVAQAIADGLAQVWPDAQLVQCPMADGGEGTVDSVLAACHGEW
RRHTVRGPLGAAVQARWGWLADSHTAIIEMAEASGLQLLPPEQRDACVTTTYGTGELIRA
ALDAGAQRIILAIGGSATNDGGAGAMQALGVQLFDAQAQRLPPGGLALSRLAHISLEQLD
PRLAQVRFEIAADVNNPLCGPHGASAIFGPQKGANPEQVQQLDAALGHFADHCARVLPND
VRDEPGSGAAGGLGFAAKAFLGAQFRAGVDVVAELVGLDEAVRGADLVITGEGRFDAQTL
RGKTPFGVARIARQHNVPVIVIAGTLGEGYEQMYAHGVDAAFALPSGPMSLEQACSEAPR
LLRERASDIARVWRLAVRTR