Protein Info for PFLU_RS14610 in Pseudomonas fluorescens SBW25-INTG
Annotation: 4-hydroxyphenylpyruvate dioxygenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to HPPD_PSEUJ: 4-hydroxyphenylpyruvate dioxygenase (hpd) from Pseudomonas sp. (strain P.J. 874)
KEGG orthology group: K00457, 4-hydroxyphenylpyruvate dioxygenase [EC: 1.13.11.27] (inferred from 100% identity to pfs:PFLU2997)MetaCyc: 94% identical to 4-hydroxyphenylpyruvate dioxygenase subunit (Pseudomonas sp. P.J. 874)
4-hydroxyphenylpyruvate dioxygenase. [EC: 1.13.11.27]
Predicted SEED Role
"4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27)" in subsystem Aromatic amino acid degradation or Homogentisate pathway of aromatic compound degradation or Plastoquinone Biosynthesis or Tocopherol Biosynthesis (EC 1.13.11.27)
MetaCyc Pathways
- L-tyrosine degradation I (5/5 steps found)
- plastoquinol-9 biosynthesis I (1/3 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
- superpathway of plastoquinol biosynthesis (2/5 steps found)
- vitamin E biosynthesis (tocopherols) (1/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.13.11.27
Use Curated BLAST to search for 1.13.11.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3KA88 at UniProt or InterPro
Protein Sequence (358 amino acids)
>PFLU_RS14610 4-hydroxyphenylpyruvate dioxygenase (Pseudomonas fluorescens SBW25-INTG) MADLYENPMGLMGFEFIEFASPTPGTLEPIFEIMGFSKVATHRSKNVHLYRQGEINLILN NEPNSIASYFAAEHGPSVCGMAFRVKDSQKAYNRALELGAQPIHIETGPMELNLPAIKGI GGAPLYLIDRFGEGSSIYDIDFVYLEGVERNPQGAGLKVIDHLTHNVYRGRMAYWANFYE KLFNFREARYFDIKGEYTGLTSKAMSAPDGMIRIPLNEESSKGAGQIEEFLMQFNGEGIQ HVAFLSDDLVKTWDALKKIGMRFMTAPPDTYYEMLEGRLPNHGEPVDQLQARGILLDGSS VAGDKRLLLQIFSETLMGPVFFEFIQRKGDDGFGEGNFKALFESIERDQVRRGVLATD