Protein Info for PFLU_RS14300 in Pseudomonas fluorescens SBW25-INTG

Annotation: response regulator transcription factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 217 PF00072: Response_reg" amino acids 8 to 118 (111 residues), 86.7 bits, see alignment E=2.4e-28 PF08281: Sigma70_r4_2" amino acids 152 to 193 (42 residues), 29.9 bits, see alignment 6.8e-11 PF04545: Sigma70_r4" amino acids 153 to 196 (44 residues), 25.5 bits, see alignment 1.5e-09 PF00196: GerE" amino acids 153 to 206 (54 residues), 58.8 bits, see alignment E=6.3e-20

Best Hits

Swiss-Prot: 37% identical to LIAR_BACSU: Transcriptional regulatory protein LiaR (liaR) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2938)

MetaCyc: 37% identical to DNA-binding transcriptional dual regulator NarL (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Two-component response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KB26 at UniProt or InterPro

Protein Sequence (217 amino acids)

>PFLU_RS14300 response regulator transcription factor (Pseudomonas fluorescens SBW25-INTG)
MSRLLRLVLADDHEVTRTGFVALLAGHPQFEVVGQARDGQQAVELCEQLLPDIVILDIRM
PVLNGLGAARLLQQRLPAIKVVIFTMDDSPDHLEAAMNAGAVGYLLKDASRNEVIEALQR
VAAGEEALNTAVSARLLRRMTERNAAGSLPTEHLTPRERQVLGLVANGMSNRQIGEHLGI
TTGTAKAHVERVIGKLGAADRTQAAVRGIALGLVTQS