Protein Info for PFLU_RS14270 in Pseudomonas fluorescens SBW25-INTG

Annotation: sterol desaturase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 175 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 37 to 57 (21 residues), see Phobius details amino acids 73 to 97 (25 residues), see Phobius details amino acids 102 to 121 (20 residues), see Phobius details PF04116: FA_hydroxylase" amino acids 40 to 164 (125 residues), 67 bits, see alignment E=1.2e-22

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2932)

Predicted SEED Role

"probable fatty acid hydroxylase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KB20 at UniProt or InterPro

Protein Sequence (175 amino acids)

>PFLU_RS14270 sterol desaturase family protein (Pseudomonas fluorescens SBW25-INTG)
MAASNKWLMSSLAYYLDFFTAPLFVILAIYLAPFDPALAIAGLLAWSLVEYSAHRFLFHS
LYRREHWTHHIDVLAYIGVSSWKTSSTFAALLLVAWYSGLTSAFIGVVAGYFYYISVHYV
MHRPAHWAYRFMPTLVANHDLHHRQGVEKNFGVSSPLWDHVFGTYVRAEPLTHYD