Protein Info for PFLU_RS13485 in Pseudomonas fluorescens SBW25-INTG

Annotation: 1,4-alpha-glucan branching protein GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 745 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 120 to 741 (622 residues), 924.9 bits, see alignment E=1.2e-282 PF02922: CBM_48" amino acids 136 to 218 (83 residues), 82.9 bits, see alignment E=2.5e-27 PF00128: Alpha-amylase" amino acids 289 to 364 (76 residues), 29 bits, see alignment E=1.3e-10 PF02806: Alpha-amylase_C" amino acids 645 to 742 (98 residues), 92.5 bits, see alignment E=2.5e-30

Best Hits

Swiss-Prot: 85% identical to GLGB_PSEPF: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to pfs:PFLU2763)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAX0 at UniProt or InterPro

Protein Sequence (745 amino acids)

>PFLU_RS13485 1,4-alpha-glucan branching protein GlgB (Pseudomonas fluorescens SBW25-INTG)
MSFTNKEPLQAKLTALPASKDVEALVRAEHHDPFSILGPHDDEHGGQFIRAFLPEALSVH
VLARDSGEQLGSLDASQVPGLFVGQFKTREPYLLKIQWAGGEQITEDPYSFQQLLLGEMD
LYLFGEGNHRDLSSCLGAQVTSVDGVQGVRFAVWAPNARRVSVVGDFNIWDGRRHPMRLR
HPSGVWEIFIPRLQPGAAYKYEILGANGILPLKADPMALATQLPPDTASKVAEPLQVDWQ
DHAWMQSRVDKQKPTAPLSIYELHVGSWQCELDEAGEVARQYNWRELAERLVPYVQQLGF
THVELMPIMEHPFGGSWGYQALSQFAPTARFGSPEDFAYFVDALHQADIGVILDWVPAHF
PTDTHGLAQFDGTALYEYANPLEGFHQDWDTLIYNLGRTEVHGYMLASALHWLKHFHIDG
LRVDAVASMLYRDYSRKAGEWVPNRHGGRENLEAIDFLRHLNDVVALEAPGALVIAEEST
AWPGVSQPTQQGGLGFNYKWNMGWMHDSLHYIQQDPVYRAHHHNELSFGLVYAWSERFIL
PISHDEVVHGKHSLIDKMPGDRWQKFANLRAYLAFMWMHPGKKLLFMGCEFGQWREWNHD
QQLDWYLLQYAEHKGVQKLVGDLNRLYREEPALHEQDDAPQGFQWLIGDDAINSVYAWLR
WSKDGKPVLVVANFTPVPREGYKVGVPFAGRWSEVINSDADMYAGSNYGNGGEVFTQDEP
NHGQAVSLALNLPPLGVLILRPEPL