Protein Info for PFLU_RS13445 in Pseudomonas fluorescens SBW25-INTG

Annotation: bifunctional diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 40 to 64 (25 residues), see Phobius details amino acids 74 to 97 (24 residues), see Phobius details amino acids 104 to 126 (23 residues), see Phobius details amino acids 140 to 161 (22 residues), see Phobius details amino acids 173 to 194 (22 residues), see Phobius details amino acids 219 to 237 (19 residues), see Phobius details PF03707: MHYT" amino acids 52 to 110 (59 residues), 74.9 bits, see alignment 6.3e-25 amino acids 115 to 167 (53 residues), 70.9 bits, see alignment 1.1e-23 amino acids 182 to 245 (64 residues), 37 bits, see alignment E=4.2e-13 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 259 to 421 (163 residues), 147.1 bits, see alignment E=2e-47 PF00990: GGDEF" amino acids 263 to 418 (156 residues), 149.8 bits, see alignment E=9e-48 PF00563: EAL" amino acids 439 to 674 (236 residues), 246.9 bits, see alignment E=2.8e-77

Best Hits

Swiss-Prot: 64% identical to Y1727_PSEAE: Uncharacterized signaling protein PA1727 (PA1727) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2753)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAW1 at UniProt or InterPro

Protein Sequence (698 amino acids)

>PFLU_RS13445 bifunctional diguanylate cyclase/phosphodiesterase (Pseudomonas fluorescens SBW25-INTG)
MLIGSYSPSLVVISLFVAILASYTALDLSGRIATAKGRGVYLWITGGAMAMGVGVWSMHF
IGMLALRLPFALGFDVGITALSLLIAVLSSGFALWLVSQPRLPAWQLALGALIMGTGIAS
MHYTGMAAMRMTPGIDYDPALFGASLLIAVAASGAALWIAYNLRRNTPYVRLARGGAAVV
MGFAIVGMHYTGMAAARFADESFCGAALNGLSGKGLDNLVLVTTLAVLAIALLTSLLDAR
LEARTAVLADSLTQANQELTHLALHDMLTGLPNRTLLADRIQQAMQAVKEQGGCFALMFI
DLDGFKPVNDAFGHHMGDQLLSEVGVRLREDLRSQDTLARIGGDEFVLLVQLTQPDDALG
LAERQVVLINRAFRVAEHELKISASVGIAMFPGNGNSPQELLMNADAAMYHAKGMGKNGY
SFFDASMNTNARKQLQLLQDLRNAVEQQQFRLYYQPKFDALSGIPVGAEALLRWEHPQQG
LLLPAMFIDMAEKTGLIIPIGEWVLNEACRQMSLWYAEGYEHWRIAVNLSALQFCHVGLV
KSVAAALERHQLPANSLTLEITETTAMSDADASMTVLQQLSDMGVDLSIDDFGTGYSSLM
YLKRLPANELKIDRGFVRDLEHDSDDAAIVSAIVALGQALGLRIVAEGVETDVQQSFLTR
LGCNSLQGYLLGHPLPADVFMADIVRAENAVAPDKSRA