Protein Info for PFLU_RS13400 in Pseudomonas fluorescens SBW25-INTG

Annotation: sugar ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 14 to 40 (27 residues), see Phobius details amino acids 76 to 97 (22 residues), see Phobius details amino acids 107 to 127 (21 residues), see Phobius details amino acids 157 to 180 (24 residues), see Phobius details amino acids 215 to 235 (21 residues), see Phobius details amino acids 265 to 287 (23 residues), see Phobius details PF00528: BPD_transp_1" amino acids 103 to 291 (189 residues), 44.9 bits, see alignment E=5.8e-16

Best Hits

Swiss-Prot: 36% identical to Y4OQ_SINFN: Probable ABC transporter permease protein y4oQ (NGR_a02190) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K10228, sorbitol/mannitol transport system permease protein (inferred from 100% identity to pfs:PFLU2744)

MetaCyc: 94% identical to polyol ABC-type transporter permease component MtlF (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, permease component 1" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAV2 at UniProt or InterPro

Protein Sequence (296 amino acids)

>PFLU_RS13400 sugar ABC transporter permease (Pseudomonas fluorescens SBW25-INTG)
MNTPAKNRLANPGWFLVSPSVALLLLWMIVPLGMTLYFSLIRYNLLYPGEDQFVGLENFT
YFVTDSGFLPGATNTLLLVGSVLLISVVFGVLISALLEASEFFGRGLVRVLLISPFFIMP
TVGALIWKNLIFHPVSGILAAVWKFFGAEPVDWLAHYPLLSIIIIVSWQWLPFAILLLMT
AMQSLDQEQKEAARLDGAGAIAIFWHLTLPHLARPIAVVVMIETIFLLSVFAEIFTTTNG
GPGYASTNLAYLIYNQALVQFDVGMASAGGLIAVVIANIAAIILVRMIGKNLTDKP