Protein Info for PFLU_RS13290 in Pseudomonas fluorescens SBW25-INTG

Annotation: response regulator transcription factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 PF00072: Response_reg" amino acids 4 to 114 (111 residues), 98.6 bits, see alignment E=2.5e-32 PF00486: Trans_reg_C" amino acids 151 to 226 (76 residues), 84.8 bits, see alignment E=3.5e-28

Best Hits

Swiss-Prot: 42% identical to AFSQ1_STRCO: Transcriptional regulatory protein AfsQ1 (afsQ1) from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

KEGG orthology group: K02483, two-component system, OmpR family, response regulator (inferred from 100% identity to pfs:PFLU2722)

Predicted SEED Role

"Transcriptional regulatory protein RstA" in subsystem Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAT1 at UniProt or InterPro

Protein Sequence (233 amino acids)

>PFLU_RS13290 response regulator transcription factor (Pseudomonas fluorescens SBW25-INTG)
MPNILLVEDDAALSELIASYLERNGYQVSVLSRGDHVRERARLNPPDLVILDLMLPGLDG
LQVCRLLRADSAGLPILMLTARDDSHDQVLGLEMGADDYVTKPCEPRVLLARVRTLLRRS
SLSEPQAANDQIIMGNLCIDLSERTVTWREQSVELSSGEYNLLVVLARHAGEVLSRDQIL
QRLRGIEFNGTDRSVDVAISKLRRKFDDNAGEARKIKTVWGKGYLFSRSEWEC