Protein Info for PFLU_RS13280 in Pseudomonas fluorescens SBW25-INTG

Annotation: multidrug efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1030 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 339 to 358 (20 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details amino acids 393 to 412 (20 residues), see Phobius details amino acids 435 to 458 (24 residues), see Phobius details amino acids 472 to 496 (25 residues), see Phobius details amino acids 539 to 556 (18 residues), see Phobius details amino acids 867 to 886 (20 residues), see Phobius details amino acids 894 to 913 (20 residues), see Phobius details amino acids 919 to 941 (23 residues), see Phobius details amino acids 968 to 988 (21 residues), see Phobius details amino acids 999 to 1022 (24 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1021 (1021 residues), 1206.8 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1027 (1027 residues), 1512.5 bits, see alignment E=0 PF03176: MMPL" amino acids 343 to 495 (153 residues), 31.8 bits, see alignment E=7.6e-12 amino acids 775 to 1019 (245 residues), 20.6 bits, see alignment E=1.9e-08

Best Hits

Swiss-Prot: 50% identical to SRPB_PSEPU: Solvent-resistant pump membrane transporter SrpB (srpB) from Pseudomonas putida

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 63% identity to atu:Atu3202)

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAS9 at UniProt or InterPro

Protein Sequence (1030 amino acids)

>PFLU_RS13280 multidrug efflux RND transporter permease subunit (Pseudomonas fluorescens SBW25-INTG)
MPQFFIDRPIFAWVVALFILLAGALAIPQLPVAQYPNVAPPKVEIYAVYPGASAQTLDES
VVSLIEQELNGADHLLYFESQSSLGSATITATFQPGTNPEMAQVDVQNRLKAVEPRLPQA
VTQQGLQVEKVSAGFLLLVTLTSNDGKLDDVALSDYLARNVMNELKRLDGVGKAQLYGAE
RAMRIWIDPQKLIGFNLTPADVNAAISAQNAQVSAGSIGDLPGTNSQEITAAVLVKGQLS
TPAEFADIVLKANPDGSTVRIGDVARVEVGSQEYQFSTRLNGKPSTAVSVQLSPGANALN
TATRVRAKMDELSRFFPANVEYKIPYDTSPFVKVSITKVVYTLLEAMALVFAVMFLFLQN
VRYTLIPTLVVPIALMGTFATMLLLGFSINVLTMFGMVLAIGILVDDAIVVVENVERIMA
TEGLSPKDATKKAMGQITGAIVGITLVLVAVFLPMGFMAGSVGVIYQQFSLSMATSILFS
AFLALTLTPALCATLLKPIAKGEHHAKGGFFGGFNKRFEQLTDRYEGWVAYALKRSGRYL
LIYLMLLVGLGWMFSRLPSSFLPVEDQGYTITDIQLPPGASKNRTVQVAEQIEAHNAGEP
GVGDTTMIMGFSFSGSGQNAALAFTTLKDWSERSSDDSASSIADRANMAFSELKDAIAYA
ILPPPVDGLGTSSGFEFRLQDRGGVGHAALMAARTELLAAAAKSPILANVRESALAEAPQ
VQLEVDRKQANALGVSFADVGNVLSSAIGSAYINDFPNQGRMQRVVVQAEGDQRSQVADL
MKINVRNNAGKMVPLSAFVEAKWTQGPTQLTRYNGYPAIAISGEAAPGHSTGEAMDEIQR
LVSQLPAGLGQEWTGLSLQERLSGAQAPLLLGLSLLIVFLCLAALYESWSIPTSVLLVVP
LGVLGAVVAVSLRGMPNDVFFKVGLITIIGLSAKNAILIIEFAKDLYDQGEDLITATLKA
ARLRLRPIIMTSLAFILGVVPLAIATGASSASQQAIGTGVIGGMITATLAVVFVPVFFVV
VMKLVKKGPK