Protein Info for PFLU_RS13250 in Pseudomonas fluorescens SBW25-INTG

Annotation: sugar kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF00294: PfkB" amino acids 5 to 303 (299 residues), 203.1 bits, see alignment E=3.3e-64

Best Hits

Swiss-Prot: 54% identical to KDGK_BACSU: 2-dehydro-3-deoxygluconokinase (kdgK) from Bacillus subtilis (strain 168)

KEGG orthology group: K11441, dehydrogluconokinase [EC: 2.7.1.13] (inferred from 100% identity to pfs:PFLU2714)

MetaCyc: 79% identical to 2-ketogluconokinase (Pseudomonas fluorescens)
Dehydrogluconokinase. [EC: 2.7.1.13]

Predicted SEED Role

"2-ketogluconate kinase (EC 2.7.1.13)" in subsystem 2-Ketogluconate Utilization (EC 2.7.1.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAS3 at UniProt or InterPro

Protein Sequence (313 amino acids)

>PFLU_RS13250 sugar kinase (Pseudomonas fluorescens SBW25-INTG)
MSEIDILSFGETMAMFVAEHTGDLAQVGQFHKRIAGADSNVAIGLSRLGFNVAWLSRVGN
DSLGRFVVDTLSKEGLDCRHVAVDPLHPTGFQLKSREDAGDDPQVEYFRKGSAASHLSVA
AISPALLKARHLHATGIPPALSEATRELSVELMTQMRKAGHSVSFDPNLRPSLWANQQTM
IREINALAGLADWVLPGLGEGRLLTGLDDPADIAAFYLDQGAEAVAIKLGPDGAYYRTQM
DQGFVAAVRVENVVDTVGAGDGFAVGMISALLENLSFREAVQRGNWIGSRAVQSRGDMEG
LPKRSELPSRSVA