Protein Info for PFLU_RS13230 in Pseudomonas fluorescens SBW25-INTG

Annotation: purine permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 transmembrane" amino acids 28 to 54 (27 residues), see Phobius details amino acids 60 to 78 (19 residues), see Phobius details amino acids 89 to 108 (20 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 148 to 170 (23 residues), see Phobius details amino acids 182 to 201 (20 residues), see Phobius details amino acids 208 to 228 (21 residues), see Phobius details amino acids 250 to 272 (23 residues), see Phobius details amino acids 338 to 361 (24 residues), see Phobius details amino acids 367 to 388 (22 residues), see Phobius details amino acids 396 to 417 (22 residues), see Phobius details amino acids 424 to 446 (23 residues), see Phobius details TIGR00801: uracil-xanthine permease" amino acids 22 to 444 (423 residues), 373.1 bits, see alignment E=1.8e-115 PF00860: Xan_ur_permease" amino acids 28 to 415 (388 residues), 293.2 bits, see alignment E=1.3e-91 TIGR03173: xanthine permease" amino acids 32 to 447 (416 residues), 440.9 bits, see alignment E=3.9e-136

Best Hits

Swiss-Prot: 49% identical to XANQ_ECOLI: Xanthine permease XanQ (xanQ) from Escherichia coli (strain K12)

KEGG orthology group: K03458, nucleobase:cation symporter-2, NCS2 family (inferred from 100% identity to pfs:PFLU2710)

MetaCyc: 49% identical to xanthine:H+ symporter XanQ (Escherichia coli K-12 substr. MG1655)
RXN-5076

Predicted SEED Role

"Xanthine permease" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAR9 at UniProt or InterPro

Protein Sequence (475 amino acids)

>PFLU_RS13230 purine permease (Pseudomonas fluorescens SBW25-INTG)
MSDAQAPRPRYKSDLIYGLEDRPHFTAAVFAALQHVLASFVGIITPTLIVGGVLGLESEV
PYLVSMALFVSGLGTFVQARTFGPIGSGLLCLQGTSFSFISVILSAGFMVKARGGGTDEI
LSTIFGICFFAAFIEVALSQFIGKLRKLITPVVTGTIITLMGLSLIKVAVTDMAGGFGAS
DLGAASNMGLAALVLLTIVVLNRFNNPFLRLGSIVIGLTLGFVVAWLLGRVDMAALPQVP
LISVPVPFKYGFSFDWVAFIPVAVIFLISPLEAAGDLTANSMISQQPVKGPLYIKRIKSG
LLADGLNSAMAATFNSLPMVTFAQNNGVIQLTGVASRYVAYFIAGLLVLLGLFPMIGAVL
QLMPKPVLGGATLIMFGTVAVAGIKILAEAGLHRRNVLIVAISLGMGLGVAAVPEVLREL
PKALHNIFESPITVGAFCAILLNIFLPEEFIELEEDEFDPESSTLKVMQDPDVTK