Protein Info for PFLU_RS13045 in Pseudomonas fluorescens SBW25-INTG

Annotation: AAA domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 PF00158: Sigma54_activat" amino acids 33 to 149 (117 residues), 25.1 bits, see alignment E=3.2e-09 PF07728: AAA_5" amino acids 33 to 163 (131 residues), 51.8 bits, see alignment E=2.2e-17 PF00493: MCM" amino acids 34 to 156 (123 residues), 33 bits, see alignment E=8.8e-12 PF01078: Mg_chelatase" amino acids 81 to 150 (70 residues), 27.3 bits, see alignment E=5.8e-10 PF17863: AAA_lid_2" amino acids 228 to 290 (63 residues), 63.9 bits, see alignment E=2.4e-21

Best Hits

KEGG orthology group: K03405, magnesium chelatase subunit I [EC: 6.6.1.1] (inferred from 100% identity to pfs:PFLU2671)

Predicted SEED Role

"ChlI component of cobalt chelatase involved in B12 biosynthesis" in subsystem Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9S7 at UniProt or InterPro

Protein Sequence (332 amino acids)

>PFLU_RS13045 AAA domain-containing protein (Pseudomonas fluorescens SBW25-INTG)
MTDIPHFPLSAVVGADDLKLALCLTAIDPKIGGVLIEGPRGMAKSTLARGLADLLASGQF
VTLPLGATEERLVGTLDLDAALGEGRAQFSPGVLAKADGGVLYVDEVNLLPDHLVDLLLD
VAASGTNLIERDGISHRHSARFVLIGTMNPEEGELRPQLLDRFGFNVALSGQTLPTERGQ
IIRRRLDFDSDPAVFCVQWSEPQAALRERCTQARARLDSIELDDRALAQITERCFAAGVD
GLRADLVWLRGARAHAAWRGANAIAEEDIEAVAEFALRHRRQEQAPPSSPPPEGQAPKPS
DTSPGQGQWGDMPAPALPTGARRDVPAWPKKP