Protein Info for PFLU_RS12850 in Pseudomonas fluorescens SBW25-INTG

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 PF00989: PAS" amino acids 23 to 116 (94 residues), 28.4 bits, see alignment E=5.8e-10 PF08448: PAS_4" amino acids 26 to 116 (91 residues), 26.6 bits, see alignment E=2.4e-09 PF14532: Sigma54_activ_2" amino acids 159 to 330 (172 residues), 80.7 bits, see alignment E=5.1e-26 PF00158: Sigma54_activat" amino acids 159 to 326 (168 residues), 222 bits, see alignment E=1.6e-69 PF01078: Mg_chelatase" amino acids 169 to 270 (102 residues), 20.9 bits, see alignment E=8.1e-08 PF07728: AAA_5" amino acids 181 to 299 (119 residues), 30.2 bits, see alignment E=1.7e-10 PF02954: HTH_8" amino acids 423 to 460 (38 residues), 48.7 bits, see alignment 2e-16

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2630)

Predicted SEED Role

"Sigma-54 dependent transcriptional regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9N8 at UniProt or InterPro

Protein Sequence (466 amino acids)

>PFLU_RS12850 sigma-54-dependent Fis family transcriptional regulator (Pseudomonas fluorescens SBW25-INTG)
MNITDNLKDYQQVRGLAIQSLFEIIEQSSEGTVIVDRDANIVWMNERYAKRFGLKSADEA
IGQPCEQVISNSLLRQVVRNDQPILLDIQDTPKGPLVVMRLPIHNEAGAVIGAIGFALFD
ELRNLSPLIERYLSMQQELASTRSLLRSRQSKYNFAHFIGTSAASLEVKRRARRSASAES
PVLLLGETGTGKELLAQAIHGASTRAHKAFVSINSAAIPADLLEAEFFGTAPGAFTGADR
KGRPGKFQIAQGGTLFLDEIGDMPLPLQSKLLRVLQEKEFEPVGSNEMLHSDVRVIAATS
MDLEAAIKRGEFRADLYYRLNVLPIQVPPLRERLEDIPALSEAILEELRSQHELDHAALA
LLAQHAWPGNIRELRNVLERAALLSDDLVLNAGEIRAAIGTFTPVERGSALPAVEGESFS
AARERFDRQVIGAALRACEGNVVEAAKRLGLGRSTLYKKMVALGID