Protein Info for PFLU_RS12495 in Pseudomonas fluorescens SBW25-INTG

Annotation: macrolide transporter subunit MacA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 37 to 373 (337 residues), 164.2 bits, see alignment E=1.9e-52 PF16576: HlyD_D23" amino acids 59 to 258 (200 residues), 45.6 bits, see alignment E=1.1e-15 PF13533: Biotin_lipoyl_2" amino acids 60 to 104 (45 residues), 32.5 bits, see alignment 1.2e-11 PF13437: HlyD_3" amino acids 185 to 267 (83 residues), 36.3 bits, see alignment E=1.6e-12

Best Hits

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 100% identity to pfs:PFLU2555)

Predicted SEED Role

"Macrolide-specific efflux protein MacA" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9G4 at UniProt or InterPro

Protein Sequence (383 amino acids)

>PFLU_RS12495 macrolide transporter subunit MacA (Pseudomonas fluorescens SBW25-INTG)
MEKSTLRKLGMLAVLTVAIGLIIYTVQAPADTPQYLTATAERGDIENAVLATGLLEGIKQ
VDVGAQVSGQLKSLKVKVGDKVKKGQWLAEIDPLILQNTLRKAQVDEENLQAQRRATAAQ
LKQAKSVYERYKGLQDDESVSRQDFEDAESTFQVQQANLLSLDAQIKSAHIQIDTAKVNL
AYTRIVAPIDGDVVGIVTQEGQTVIASQLAPVLLKLADLDTMTVKAQVSEADVIHISPGQ
QVYFTILGEADKRYYAKLRGTEPAPQNFLETQAAGTPKQNTAVFYNALFDVPNPDHRLRI
AMTAQVRIVLDTAQAALMVPVAALGARNNDGSYPLRVLDAKGKAVSRDVKTGINNNVKVQ
ILDGLAEGDKVVIGDATPAVAGN