Protein Info for PFLU_RS12480 in Pseudomonas fluorescens SBW25-INTG

Annotation: non-ribosomal peptide synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 500 1000 1500 2000 2500 3000 3500 4000 4286 PF00668: Condensation" amino acids 95 to 519 (425 residues), 158.2 bits, see alignment E=6.1e-50 amino acids 1156 to 1588 (433 residues), 141.7 bits, see alignment E=6.3e-45 amino acids 2205 to 2643 (439 residues), 139.1 bits, see alignment E=3.8e-44 amino acids 3264 to 3698 (435 residues), 147.4 bits, see alignment E=1.1e-46 PF00501: AMP-binding" amino acids 540 to 887 (348 residues), 313.9 bits, see alignment E=2.7e-97 amino acids 1608 to 1936 (329 residues), 250.8 bits, see alignment 4.2e-78 amino acids 2664 to 3000 (337 residues), 261.2 bits, see alignment E=2.8e-81 amino acids 3719 to 4065 (347 residues), 327.4 bits, see alignment E=2.1e-101 TIGR01733: amino acid adenylation domain" amino acids 560 to 959 (400 residues), 458 bits, see alignment E=6.5e-141 amino acids 1629 to 2008 (380 residues), 401.9 bits, see alignment E=7.1e-124 amino acids 2685 to 3072 (388 residues), 421.5 bits, see alignment E=8.2e-130 amino acids 3740 to 4137 (398 residues), 472.5 bits, see alignment E=2.7e-145 PF13193: AMP-binding_C" amino acids 943 to 1016 (74 residues), 38.6 bits, see alignment (E = 3.5e-13) amino acids 1992 to 2065 (74 residues), 39.1 bits, see alignment (E = 2.3e-13) PF00550: PP-binding" amino acids 1045 to 1107 (63 residues), 48.1 bits, see alignment (E = 2.4e-16) amino acids 2094 to 2156 (63 residues), 47.5 bits, see alignment (E = 3.6e-16) amino acids 3153 to 3215 (63 residues), 48.9 bits, see alignment (E = 1.3e-16) amino acids 4218 to 4277 (60 residues), 47.7 bits, see alignment (E = 3e-16)

Best Hits

Predicted SEED Role

"Non-ribosomal peptide synthetase modules, pyoverdine??" in subsystem Siderophore Pyoverdine

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9G2 at UniProt or InterPro

Protein Sequence (4286 amino acids)

>PFLU_RS12480 non-ribosomal peptide synthetase (Pseudomonas fluorescens SBW25-INTG)
MQFSELLAAISTHAIRLQLEEGDLIILGDDEALDDALWDQLIAHKPRLLELVAEHGGDWL
SPAYRITPDMLPLVSLDQAAIDRIVAAIPGGAANVQDIYPLAPLQEGMLYHHLSAEQGDP
YVLHAHFEFDNRARLHAFTEALQWVIDRHDILRTSIVWERLDEPLQVVWRKASLACEAVD
FDARNDRMDLGQAPLLRLVFTQDSLTGCVAATLLFHHTILDHTALDVVRHEIQLYLAGEQ
AQAGEPVPFRSYIAQVRHGVSEQAHEVFFREMLADIEEPTLPFGLQDVQGDGLGIDEARL
AVDSALSRRLRTLARPLGVSVASMMHLALARVLGVVSGRDAVVFGTVMLGRMGAGEGGER
ALGMFINTLPLRVDVGEQSVRAGVKATHERLTTLLNHEHASLALAQRCSGVAAPTPLFSA
MLNYRHSAATDMAEVIEIAEGIRVLSAEERTNYPLTVNVDDLGEDFALTVMVDASIGAER
VATYLHTALESLAEALEQRPDMPLSGLTILPDTERQSLLHRFNHSPALYADTALIHQHVE
THAAAQPEAIALRFDNQRLTYRQLNERANQVAHRLLAQGIRPDDRVAICVERGPEMIIGL
LGILKAGAGYVPIDPAYPLERIAYTLTDSEPLAVLVQANTRHLVGDLPQVDLNGLRGESI
VNPRVNVSPAHLAYVIYTSGSTGQPKGVMIEHRQVARLFTATEHWFDFNRNDVWALFHSF
AFDFSVWEIWGALMHGGQLLIVPQLVSRSPDECYTLLCEAGVSILNQTPSAFRQLIAAQG
QNPQSHSLRQVIFGGEALEPGMLKPWYARAINAGTQLVNMYGITETTVHVTYRALEAADA
QLVGVSPIGVRIPDLQLYVLDARREPLPLGVVGELYVGGAGVARGYLNRDALTAERFLPD
PATGLRMYKTGDLGRLLADGSVEYLGRNDDQVKIRGFRIELGEIEAHLASAEGVRDAVVV
AREDAPGDKRLVAYVIAEGALRAADLRDHLLLSLAEHMVPSAFVQLDQFPLTTNGKLDRK
ALPAPDADALARRGYEAPRGAVENAIAAIWQDLLKLEHVGRDDNFFELGGHSLLAVKLIE
RMRQVDLSADVRVLFGQPTLAALAAAVGGQQEVQVPTNLITHATTHITPDMLPLVELDQA
ALDHIIQRVPGGISNVQDIYGLAPLQAGILYHHLATTEGDPYVLQVQFSFNDQAAVDAFI
QALHSVIERNDILRTAILWEGLNEPVQVVLRQAALAVERIDEPFEQLQQRFDPRHFRLDL
SRASLMRFAYAEEQGRFVGILLLHHILLDHTALQVLVAEMSASLSGNSAQLPDAVQYRNY
VAQARLGVSQAQHEAFFSEMLGDIDEPTLAFGLQDVNGDGSGILEAHLPLEPALSLGLRE
QARQLGVSTASLVHLAWAQVLGQVSGQQAVVFGTVLLGRMQGGEGADRALGMFINTLPLR
VSLGSVGVQAGVRATHARLAQLLGHEHASLSLAQRCSGVPGSLPLFSTLLNYRHSAPGEA
PGHSPFAASGIQILSSEERSNYPLVLNVDDLGTGFALTVQGVASLDVQRVGDYMLTALRH
LVTALQQAPTTPLQAISILPAAERHQLLVDFNATARDYPAHLTVHQLFEAQALARPEAVA
AVHGPLSLSYRDLNRRANRLAHHLLNQGVQPGESVAIALPRSLDLLICQLAILKCAAVYV
PLDVNAPVERQAFIVQDSGAHRVLSSLVDLNLDGLSPRNPELPQSSESVAYILYTSGSTG
APKGVQVPHRAISRLVLNNGYAEFNARDRVAFASNPAFDASTLDVWAPLLNGGCVVVVEH
AVLLSQAAFAALLQEQSVSVLWMTAGLFHQYADALMPVFHQLRYLIVGGDVLDPQVIGRV
LEHGKPQHLLNGYGPTEATTFSTTFEITRVGEGGIPIGKPIANAQAYVLDARQQPVPLGV
VGELYIGGAGVATGYLNQPQLTAEKFIPNPFGDGVLYRTGDLACWQADGTLLYQGRNDLQ
VKIRGFRIEPGEIETCLASFPGVKDTVVLAREDEPGDKRLVAYYTAGAALEIEALRAHLQ
GQLPDYMVPSAYVWLELLPLTANGKLDRKGLPVPDQSALLSRGFEAPEGEVETQLAQIWQ
DVLKLERVGRHDHFFELGGHSLLAVSLIERMRQVGLSCDVRVLFNQPSLAALAAAVGSGR
EVVVPANGIPLGCTQITPSMLTLVQLDPAAIERIVANVPGGAANVQDIYPLAPLQEGILY
HHISAEQGDPYLLQSRMAFDSLERLHGFMGALQQVVARHDILRTGVVWEGLDSPVQVVWR
GAQLRVQEVALDPADGGIIEQLHARFDARHYRLDITQAPLLRMVYAQDPQHNRVAAILLF
HHLALDHTAMAVVGQEMRAFMFNQAQDLPVAAPFRNYVAQARLGVSVEEHEAFFRDMLAD
VEEPTLPFGVQDVQGDGRDIEEAEQPVDATLARRVREQARQLGVSAASLMHLAWAQVLGL
VSGRDDVVFGTVLMGRMQAGDGADRALGMFINTLPLRVDVAATAAAAVKATHGRLSALLG
HEHASLALAQRCSGVANASPLFSALLNYRHSSPDEMARDGHGIWEGVQLLGGEERSNYPL
TLSVDDLGEGFGLTVLALPQIGAQRLCAYMHNAVEQLVAALESAPQTALNQLPMLAATER
ETLLRDFNATVHDFPQGQTVHGAFEVQAERQPQAVAVLQGGEALTYEQLNQRANQLAHHL
LALGVQPDDRVALCCRRGPQMLVGLLGILKAGAGYVPIDPAYPAERIAYLLHDSAPVVAV
AEASTRALLGEVASVDLHDPRLQHLAVTNPPLDTLTPAHLAYVIYTSGSTGQPKGVMVEH
RTVENLVNWHCEAFGLGATGHTSSVAGFGFDAMAWEVWPALCVGATLHLPPANVGNENID
ELLAWWLAQPLDVSFLPTPVAEYAFSQQLQHPTLRILLIGGDRLRQFTHERRFAVINNYG
PTEATVVATSGRVRAGQVLHIGRPIANASVYLLDAQLRPVPVGVTGELYVGGSGVARGYL
NRPDLTAERFLQDPFNAGRMYRTGDLARWLPDGNIEYLGRNDDQVKLRGVRVELGEIESR
LAALDGVGEAVVLVREGRLIAWFTAQQPLDIDTLRTQLQAQLPDALVPVAYVKLHALPLT
ANGKLDRKALPEPDHAALLTRVYEAPQGEVETTLARIWAEVLHVEQVGRHDHFFELGGHS
LLAVSLIERMRQVGLSADVRVLFSQPTLAALAAAVGSGREVQVPVNRIPADCRRITPDLL
TLVELDQATIDQVVAQVPGGTANVQDIYPLAPLQEGILYHYITAEQGDPYLLQTHLAFDS
LERLNAFTQALQQVIDRHDILRTGVVWEGLAQPLQVVWRTAELQVQALHLQGDVLAGLHE
RFDARRYRLDISQAPLIRLMYAQDPAHQRVVAVLLYHHIVLDHTAFDVVLREMQGFLLGR
QVPAAAPMPYRNYVAQARLGVSEQEHEGFFRAMLGDIDEPTLPFGWQDVRGDGSAIEEHS
LRLHSDLNRRLRAQARLMGVSTASLFHLAWGQVLAATSGRHSVVFGTVLVGRLQGGEGAD
RALGVFINTLPLRLDIDGQGARAAVRATHERLSALLGHEHASLALAQRCSGVAAPAPLFS
SMLNYRHRGAATRSQEAQQAWEGMQTLVNDGRTNYPLTLNVDDLGDGYDITALARVDAQR
VCDYLQTALSGLVEALEHAPQHALDRLPILGAEERNKLLVEFNSTEVPYDLDQTLQGLFE
AQAERTPEAVAVIAAEQRLTYQALNERANRLAHHLRERGVLPDTRVGICLERGLDMVVGL
FAILKAGGAYVPLDPAYPQERIAYMLQDSAPVVVLAQATTHALLGDVPVIELDHVTWQHQ
PATNPQVPGLTARHAAYVIYTSGSTGEPKGVINEHAGVVNRLLWMQDAYGLNAEDAVLQK
TPFSFDVSVWEFFWPLFTGARLVMARPGGHKDPAYLCEVIEAQRITTLHFVPSMLDVFLA
HGDVSQAEGLVRVMCSGEALPGSLVRRFKQQLPGIGLYNLYGPTEAAVDVTAWNCARPEV
PDNTPIGKPIANTRMYVLDAQLQPVPLGVVGELFIGGVQVARGYLNRAQLTAERFLKDPF
TGGRLYRTGDVGRYLPDGNIEYLGRNDDQVKIRGLRIELGEIQARLSEHASVKDAVVVAR
DERLVAYYTGEHTPVESLRAQLLLHLPDFMVPALFVHLDALPLSPNGKLDRKALPAPGMD
ARRVRAFEAPEGDTEILLARLWAELLNVERVGRHDNFFELGGHSLLAVSLIGRLRQEGME
ADVRALFEQPTLAGYAALTERMEIVL