Protein Info for PFLU_RS12410 in Pseudomonas fluorescens SBW25-INTG

Annotation: MATE family efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 52 to 75 (24 residues), see Phobius details amino acids 89 to 113 (25 residues), see Phobius details amino acids 133 to 152 (20 residues), see Phobius details amino acids 164 to 185 (22 residues), see Phobius details amino acids 192 to 213 (22 residues), see Phobius details amino acids 244 to 267 (24 residues), see Phobius details amino acids 287 to 309 (23 residues), see Phobius details amino acids 322 to 344 (23 residues), see Phobius details amino acids 361 to 381 (21 residues), see Phobius details amino acids 388 to 409 (22 residues), see Phobius details amino acids 420 to 440 (21 residues), see Phobius details PF01554: MatE" amino acids 19 to 178 (160 residues), 92.5 bits, see alignment E=1.2e-30 amino acids 248 to 409 (162 residues), 100.9 bits, see alignment E=3.1e-33 TIGR00797: MATE efflux family protein" amino acids 19 to 420 (402 residues), 198.7 bits, see alignment E=7.5e-63

Best Hits

Swiss-Prot: 42% identical to YOEA_BACSU: Probable multidrug resistance protein YoeA (yoeA) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2538)

Predicted SEED Role

"Na+ driven multidrug efflux pump"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9E8 at UniProt or InterPro

Protein Sequence (451 amino acids)

>PFLU_RS12410 MATE family efflux transporter (Pseudomonas fluorescens SBW25-INTG)
MQSPLQRPLWQIYLIFLAPMVLSNFLQSFSGTLNGIYVGQMLGTQALAAVSGMFPIVFFF
IALVIGLGAGASVLIGQAWGAQETAMVKAITGATLTLGALVGVIAAVLGSLFARPALQAL
GTPVDVLDDAVGYAQKMMLIMPLLLVFILYTQLLRGVSDTISPLLALMVSTLVGLLLTPA
LIRGWVGLPPLGIQSAVYAGLVGNALAMLFLILRLRHKNHVMAPDRELLAALRLDRVILG
KVLRIGLPTGLQMVVLSLSELVILALVNGHGSQATAAYGAVTQIVNYVQFPALSIAITAS
ILGAQAIGAGRLERIGPILRTGLLINTCLTGGLIVLGYVLSHWLLGLFITDDAARVNAEH
LLHIMLWSILVFGFQAVIGGIMRASGVVLMPVVISIFCVLCVELPMAYLLNAHFGLEGVW
MAFPVTYLAMLVLQTAYYRLVWRHKQIKRLV