Protein Info for PFLU_RS12015 in Pseudomonas fluorescens SBW25-INTG

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 559 (558 residues), 421.4 bits, E=3.4e-130
• PF13522: GATase_6
  • amino acids 33 to 162 (130 residues), 103.8 bits, E=1.1e-33
• PF13537: GATase_7
  • amino acids 51 to 168 (118 residues), 123.8 bits, E=6.4e-40
• PF00733: Asn_synthase
  • amino acids 240 to 605 (366 residues), 283.6 bits, E=5.4e-88

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to pfs:PFLU2452)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (8/8 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See C3K8M3 at UniProt or InterPro

Protein Sequence (634 amino acids)

>PFLU_RS12015 asparagine synthase (glutamine-hydrolyzing) (Pseudomonas fluorescens SBW25-INTG)
MCGYIGVFAKQPRAFNPNLFDAALRAIHHRGPDSSSQWFDPKGQAAFGYVRLGLVGLGNG
TQPIVADEGDLVMMVNGEFYDYQRIRTELEDYGCRFKTSSDSEIAMHLYRRHGVRGLKQL
RGEFSILIFDRLRKKLFAVRDRVGVKPLYYTEHEGAWYFASEIKALLAAGVPAEWDHEAY
ANRGFILRDRTVFNNIRSVKPGCWIIADESGLQTEQYWDWDFPDAQATEQRSEAEMIDSL
RHTIEESVRLRLHADVPVGVCLSGGLDSSAMLGIATELTGQPLQAFHLSFEGEQAYDERQ
YAEIAARHNRAHLNVLSVNSADMADNFENALWHAEMPFANAHSVAKYLLCKYVQNQGMRA
VLTGEGADEVFGGYPHYRRDMVLYNHENQDPTAIAELARRLHASEDRYLPGGKNDVKWVQ
DELGHGVSWLQTQSALFGPLAQLYSDDFRERFSNTDAYREFYDRLSPRALNGWEPVNRSL
YMVAKSSLPNVVLTSLGDRMEMAGSLEGRPPLLDHQVIEAACRLPVNMKVRGATEKYALR
EAMRPYVPQAVLDRKKQYFRAPPASESPQSKLYEMINDVLSGPALSNVPFFEPRKVRALL
ATLPSLSPAQRASADNLLMEIAGLCLMQKRFALN