Protein Info for PFLU_RS11570 in Pseudomonas fluorescens SBW25-INTG

Annotation: hydroxypyruvate isomerase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 PF01261: AP_endonuc_2" amino acids 21 to 256 (236 residues), 176.4 bits, see alignment E=4.4e-56

Best Hits

Swiss-Prot: 73% identical to OTNI_CUPNH: 2-oxo-tetronate isomerase (otnI) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K01816, hydroxypyruvate isomerase [EC: 5.3.1.22] (inferred from 100% identity to pfs:PFLU2356)

MetaCyc: 48% identical to hydroxypyruvate isomerase (Escherichia coli K-12 substr. MG1655)
Hydroxypyruvate isomerase. [EC: 5.3.1.22]

Predicted SEED Role

"Hydroxypyruvate isomerase (EC 5.3.1.22)" (EC 5.3.1.22)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.22

Use Curated BLAST to search for 5.3.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K8J5 at UniProt or InterPro

Protein Sequence (261 amino acids)

>PFLU_RS11570 hydroxypyruvate isomerase family protein (Pseudomonas fluorescens SBW25-INTG)
MPRFAANLSMLYPQYDFLERFSAAKADGFDAVEYLFPYEYDAQVLKQRLSDNGLVQALFN
APPGDWAAGERGIASLPGREAEFRSGFDRALEYAAVLGNDRVHVMAGLLPSENLRQRHQA
VYLENLAYASAAAAEVGVTVLLEPINTRDMPGFFLNRQDQAHAICREVGARNLKVQFDCY
HCQIVEGDLATKLRRDFDGIGHIQIAGVPDRHEPDLGELNYAYLFELMDQLGYDGWVGCE
YRPKGDTSAGLQWLRDWKGAR