Protein Info for PFLU_RS10815 in Pseudomonas fluorescens SBW25-INTG

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR01891: amidohydrolase" amino acids 36 to 420 (385 residues), 293.2 bits, see alignment E=1.5e-91 PF01546: Peptidase_M20" amino acids 93 to 427 (335 residues), 113.4 bits, see alignment E=1.5e-36 PF07687: M20_dimer" amino acids 227 to 323 (97 residues), 50 bits, see alignment E=2.6e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2199)

Predicted SEED Role

"N-acyl-L-amino acid amidohydrolase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7T9 at UniProt or InterPro

Protein Sequence (438 amino acids)

>PFLU_RS10815 amidohydrolase (Pseudomonas fluorescens SBW25-INTG)
MLKLPISLLLLSGSVSAAEPWDDLDAQISQALPGVIELRHAIHQQPELGNREFHTAERIA
QRLRELGLEVQTGIAHTGVIGILRGGKPGPVVAIRAELDALPLTEQTGLPFASTVRSKDE
GADFRSRGKDVGVMHACGHDAHMAMALGVAEVLAAHREQLPGTLKLIFQPAEEGPPLGEQ
GGAQLMIKEGALQNPAPAVIFGIHVTAGTAGTFTLSRQRTTAAADTFVARIIGRQTHAAF
PWTGIDPVPVAAQVILAWQTIPSRQSNLSLLPAPVISVGRIEAGDRHNIIPAEVRLEGSI
RTVSDEQREDVLQRMTRTAEHIAEASGATTKVDYLPGNYRAGYNNTALVDQLMPVLQQVS
TTPVRIDNGTYGADDFAEYARQVPGVFMRMGVTPPALEGKTVWPTHSPGFQVDDPSLAVG
IRALSRMTLSYMIDAPRS