Protein Info for PFLU_RS10295 in Pseudomonas fluorescens SBW25-INTG

Annotation: HAD-IA family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 234 PF00702: Hydrolase" amino acids 24 to 200 (177 residues), 82 bits, see alignment E=1.2e-26 PF13419: HAD_2" amino acids 26 to 205 (180 residues), 71.6 bits, see alignment E=1.4e-23 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 96 to 205 (110 residues), 48.7 bits, see alignment E=9.4e-17 PF13242: Hydrolase_like" amino acids 163 to 205 (43 residues), 23.4 bits, see alignment 7e-09

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2090)

Predicted SEED Role

"Putative phosphatase YieH" in subsystem 2-phosphoglycolate salvage

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5Q8 at UniProt or InterPro

Protein Sequence (234 amino acids)

>PFLU_RS10295 HAD-IA family hydrolase (Pseudomonas fluorescens SBW25-INTG)
MCLSADIGELPGQVKAAALVGVPKLIIFDCDGVLVQSEEITLSVLISMLNALASRDRLLD
VTGFIEHFRGRKIADCLREAEQILKLTLDTEFEQRFRQQALEALTLSLKATEGMVEVLES
LTVPYCVASSAPRNKIEHCLRISGLFSYFEGRIFSCYEVGRWKPDPLVFLTACDTYNVDV
SDALVIEDSVTGIQAAVAANISVIGFGPTYRHSQLVAVGAVPITDIRELLTIFN