Protein Info for PFLU_RS10140 in Pseudomonas fluorescens SBW25-INTG

Annotation: threonine/homoserine exporter RhtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 39 to 60 (22 residues), see Phobius details amino acids 72 to 91 (20 residues), see Phobius details amino acids 97 to 116 (20 residues), see Phobius details amino acids 123 to 140 (18 residues), see Phobius details amino acids 146 to 167 (22 residues), see Phobius details amino acids 179 to 200 (22 residues), see Phobius details amino acids 207 to 226 (20 residues), see Phobius details amino acids 238 to 258 (21 residues), see Phobius details amino acids 264 to 283 (20 residues), see Phobius details PF00892: EamA" amino acids 149 to 278 (130 residues), 63 bits, see alignment E=1.7e-21

Best Hits

Swiss-Prot: 59% identical to RHTA_ECOLI: Threonine/homoserine exporter RhtA (rhtA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2063)

MetaCyc: 59% identical to L-threonine/L-homoserine exporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-242; TRANS-RXN0-0244

Predicted SEED Role

"Threonine and homoserine efflux system"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K529 at UniProt or InterPro

Protein Sequence (295 amino acids)

>PFLU_RS10140 threonine/homoserine exporter RhtA (Pseudomonas fluorescens SBW25-INTG)
MTTSPRSLASTLFPVGLLLIAMASIQSGASLAKSMFPIVGAQGTTALRLIFASVIMLLLL
RPWRAKLTAKSLRTVIVYGMALGGMNFLFYMSLRSVPLGIAVALEFTGPLAVAIYASRRA
VDFLWIVLAIIGLLLLIPMGEASSGIDLLGAGYALGAGVCWALYILYGQKAGNDNGVQTA
ALGVMIAALFVAPIGIVHAGSALLTPALIPVAIGVAILSTALPYTLEMVALTRLPARTFG
TLMSIEPAFGAVSGLLFLHEHLSIAQWLAITCIILASVGATVTMRSESKPLVPAD