Protein Info for PFLU_RS09900 in Pseudomonas fluorescens SBW25-INTG

Annotation: amino acid permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 469 transmembrane" amino acids 21 to 38 (18 residues), see Phobius details amino acids 45 to 67 (23 residues), see Phobius details amino acids 100 to 123 (24 residues), see Phobius details amino acids 129 to 150 (22 residues), see Phobius details amino acids 158 to 182 (25 residues), see Phobius details amino acids 203 to 225 (23 residues), see Phobius details amino acids 247 to 267 (21 residues), see Phobius details amino acids 279 to 304 (26 residues), see Phobius details amino acids 336 to 358 (23 residues), see Phobius details amino acids 364 to 389 (26 residues), see Phobius details amino acids 409 to 427 (19 residues), see Phobius details amino acids 433 to 451 (19 residues), see Phobius details PF00324: AA_permease" amino acids 20 to 450 (431 residues), 362.8 bits, see alignment E=2.6e-112 PF13520: AA_permease_2" amino acids 24 to 443 (420 residues), 141.3 bits, see alignment E=4.6e-45

Best Hits

Swiss-Prot: 61% identical to CYCA_ECOLI: D-serine/D-alanine/glycine transporter (cycA) from Escherichia coli (strain K12)

KEGG orthology group: K11737, D-serine/D-alanine/glycine transporter (inferred from 100% identity to pfs:PFLU2013)

MetaCyc: 61% identical to D-serine/alanine/glycine:H+symporter (Escherichia coli K-12 substr. MG1655)
RXN0-5130; RXN0-5201; RXN0-5202; RXN0-5203; TRANS-RXN-62A; TRANS-RXN-62B

Predicted SEED Role

"D-serine/D-alanine/glycine transporter" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K898 at UniProt or InterPro

Protein Sequence (469 amino acids)

>PFLU_RS09900 amino acid permease (Pseudomonas fluorescens SBW25-INTG)
MKTVEIEGGDELRRGLSNRHIQLISIGGAIGTGLFMGSGKTIGVSGTSIILTYTVIGFFL
FFVMRAMGELLLSNLKYNSFTDFCSDYIGPWAGYFIGWSYWLTWVVVAIADCTVIGGYLR
FWYPELPTWIPALGVLAVLLSINLVAVRLFGEMEFWFALIKVVAIGALIVTGFIMVSISF
VSPVGVTASLSHLLEEGAVFPHGITGFFAGFQIAVFSFAGIELIGTTAAETKNPKVTLPR
AINSVPARVLFFYVLSLTCIISVSSWGEIAADKSPFVQLFILAGLPAAAGIINLVVITSA
MSAANSGVFATSRMLFSLSANGRGAKLFQKLSKSSVPVFGLIFSCLCMLIGLGLLFIIPE
VMTAFTILTTISAILFIFVWSMIVVAYLIYRKRNPELHKSSDFKMPCGIVMSWLCLLFFV
FILILLALEPDTAQALSIMPIWFLLLAGLYSRNRRKSASLSVGSEAING