Protein Info for PFLU_RS09850 in Pseudomonas fluorescens SBW25-INTG

Annotation: acetyl-CoA carboxylase biotin carboxylase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 5 to 113 (109 residues), 138.4 bits, E=2.7e-44
• PF02786: CPSase_L_D2
  • amino acids 118 to 324 (207 residues), 242.2 bits, E=8.5e-76
• PF07478: Dala_Dala_lig_C
  • amino acids 146 to 294 (149 residues), 33.6 bits, E=5.7e-12
• PF02785: Biotin_carb_C
  • amino acids 339 to 445 (107 residues), 119.7 bits, E=1.3e-38

Best Hits

Swiss-Prot: 50% identical to ACCC1_BACSU: Biotin carboxylase 1 (accC1) from Bacillus subtilis (strain 168)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to pfs:PFLU1997)

MetaCyc: 52% identical to 4-hydroxyacetophenone carboxylase biotin carboxylase subunit (Aromatoleum aromaticum EbN1)
RXN-9833

Predicted SEED Role

"Biotin carboxylase (EC 6.3.4.14)" (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 4-ethylphenol degradation (anaerobic) (2/6 steps found)
• 3-hydroxypropanoate cycle (6/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• glyoxylate assimilation (4/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See C3K7R1 at UniProt or InterPro

Protein Sequence (457 amino acids)

>PFLU_RS09850 acetyl-CoA carboxylase biotin carboxylase subunit (Pseudomonas fluorescens SBW25-INTG)
MTQAIQKLLVANRGEIAVRIIRAAKALGIPTVAACSEADVDSRAARMADEVHLLGPARAD
KSYLNVEALLGALKATGANAVHPGYGFLSENADFAEAVLAAGAIFVGPSPETIRRMGDKA
EARRTAQEAGVPVVPGSPGELFDVESALKAAEAVGFPLLIKASAGGGGRGIRLAENAEQL
AEEFPRSQREAQAAFGNGAVYLERFISRARHIEVQVLGDGQHAVHLFERECSLQRRRQKI
FEEAPSPALNPQQRETLCTSAVRLTESLGYKGAGTLEYLYDDVTGEFFFIEMNTRIQVEH
PVSELITGIDLVQAMLRIAGGEPLGFKQSDIQLNGAALQMRLNAEDPERDFFPSPGLVES
LIWPQGEGVRVDTHLYQGYRVPPYYDSLLAKLIVHGRDRAEALTRARLAVKHTTLSGMAT
TLSLHGELLEQPWLHSADFHTGTLETWLAERGRGGQA