Protein Info for PFLU_RS09435 in Pseudomonas fluorescens SBW25-INTG

Annotation: IS66 family transposase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 PF13007: LZ_Tnp_IS66" amino acids 49 to 122 (74 residues), 47.2 bits, see alignment E=6.2e-16 PF13005: zf-IS66" amino acids 130 to 171 (42 residues), 52.8 bits, see alignment 8.3e-18 PF03050: DDE_Tnp_IS66" amino acids 185 to 466 (282 residues), 354.8 bits, see alignment E=7.4e-110 PF13817: DDE_Tnp_IS66_C" amino acids 473 to 510 (38 residues), 61.8 bits, see alignment 1.1e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1911)

Predicted SEED Role

"Mobile element protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K518 at UniProt or InterPro

Protein Sequence (515 amino acids)

>PFLU_RS09435 IS66 family transposase (Pseudomonas fluorescens SBW25-INTG)
MTSSPNLDQMSPDQLRALAAQLLSQIDSLGQQVDRMGKKNDRDQTIIEQLTHEIAWFKRN
KFAKRSEQLSPAQGSLLDDLLDTDIVAIEAELKALNPPAAPAEPRQQPKRVSLPAQFPRT
VIYHEPDTTLCTCGCQLQRIGEDVSEKLDYTPGVFTVEQHVRGKWACRQCETLIQAPVPA
QVIDKGIPTAGLIAHVLVAKFADHLPLYRQEKIFGRAGLAIPRSTLAQWVGQTGVQLQPL
VDALREAVLAQQVVHADETPVQMLAPGEKKTHRAYVWAYCTTPFSALKAVVYDFSPSRAG
EHARNFLGAWNGKLVCDDFAGYKAGFEKDMIEIGCMAHARRKFFDLHVANKSQLAEQALH
SIGGLYEVERQVRDMSDEDRCRIRQEKAAPLAKALHDWMLAQRDLVPNGSATAKALDYSL
KRWVALTRYLVDGAVPIDNNPVENQIRPWALGRSNWLFAGSLRSGKRAAAIMSLIQSARM
NGHDPYAYLKDVLTRLPTQRASEIDQLLPHQWVSA