Protein Info for PFLU_RS08595 in Pseudomonas fluorescens SBW25-INTG

Annotation: sodium:proton antiporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 53 to 72 (20 residues), see Phobius details amino acids 81 to 103 (23 residues), see Phobius details amino acids 109 to 126 (18 residues), see Phobius details amino acids 147 to 166 (20 residues), see Phobius details amino acids 172 to 194 (23 residues), see Phobius details amino acids 207 to 229 (23 residues), see Phobius details amino acids 235 to 253 (19 residues), see Phobius details amino acids 267 to 285 (19 residues), see Phobius details amino acids 291 to 312 (22 residues), see Phobius details amino acids 323 to 342 (20 residues), see Phobius details amino acids 354 to 374 (21 residues), see Phobius details PF00999: Na_H_Exchanger" amino acids 6 to 370 (365 residues), 222.4 bits, see alignment E=8.9e-70 TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 10 to 281 (272 residues), 251.6 bits, see alignment E=5.1e-79 PF02254: TrkA_N" amino acids 406 to 518 (113 residues), 84.9 bits, see alignment E=5.4e-28

Best Hits

KEGG orthology group: K03455, monovalent cation:H+ antiporter-2, CPA2 family (inferred from 100% identity to pfs:PFLU1748)

Predicted SEED Role

"Glutathione-regulated potassium-efflux system protein KefB" in subsystem Glutathione-regulated potassium-efflux system and associated functions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7K9 at UniProt or InterPro

Protein Sequence (576 amino acids)

>PFLU_RS08595 sodium:proton antiporter (Pseudomonas fluorescens SBW25-INTG)
MFANLLIILASSLVVIALFRRLQLPPVLGYLCVGLAVGPTALDWVNDSEELPDLAELGVV
FLLFSLGLEFSLTKMLALRRVVFGLGSLQVLGCGTLLAVLLMGFGVAPGIALLLGAGLAL
SSTAIVSKELTSLGEIFSSHGQNAIGVLLFQDVVAVLLLTLVPVFAGTGEQAWYWALPLT
LGKTVVLFVGLLLASRWLLPRLFHEVAASHSAELFVLLALVIVLLTAWLTHLLGLSPALG
AFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSIGMLIDLQLFVSHSLLILGLTLALM
LIKGCVVAALVKLRGSDGETAWRSGLALAQGGEFCFALMAQMQQSRLIPDEFNGLLLAAT
FCSMLLTPLLLRAAPSIALRLHRKPNQQVQLEAITALNAQLHGHAVICGYGRVGQSIGRF
LRKEQQAFIALDDDPERVQETATEDSSVHYGDCRRGVLLSAVGLERARLVVIAVDNSDVA
LVVLKEARRINPDVPILVRTRDDSQLAELKAAGASEVVPELLESSLMLASHALILLGLPD
QQVQARVDEVRQNRYRLLHGFYAQPNNEDEAPINPD