Protein Info for PFLU_RS08230 in Pseudomonas fluorescens SBW25-INTG

Annotation: potassium-transporting ATPase subunit KdpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 687 transmembrane" amino acids 47 to 67 (21 residues), see Phobius details amino acids 73 to 94 (22 residues), see Phobius details amino acids 226 to 249 (24 residues), see Phobius details amino acids 259 to 283 (25 residues), see Phobius details amino acids 592 to 611 (20 residues), see Phobius details amino acids 621 to 640 (20 residues), see Phobius details amino acids 660 to 683 (24 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 24 to 682 (659 residues), 972.5 bits, see alignment E=9.3e-297 TIGR01494: HAD ATPase, P-type, family IC" amino acids 82 to 350 (269 residues), 101.7 bits, see alignment E=3.5e-33 amino acids 374 to 607 (234 residues), 149 bits, see alignment E=1.5e-47 PF00122: E1-E2_ATPase" amino acids 121 to 291 (171 residues), 104 bits, see alignment E=1.4e-33 PF00702: Hydrolase" amino acids 310 to 535 (226 residues), 86.4 bits, see alignment E=7.2e-28 PF12710: HAD" amino acids 448 to 531 (84 residues), 28.6 bits, see alignment E=3.7e-10

Best Hits

Swiss-Prot: 85% identical to KDPB_PSEAE: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01547, K+-transporting ATPase ATPase B chain [EC: 3.6.3.12] (inferred from 100% identity to pfs:PFLU1677)

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4Y4 at UniProt or InterPro

Protein Sequence (687 amino acids)

>PFLU_RS08230 potassium-transporting ATPase subunit KdpB (Pseudomonas fluorescens SBW25-INTG)
MKMNMPAKNAAPVKTQEPAKTAISALWRPALVQAFVKLDPRQLQRSPVMLVVELTAILTT
VLCFVPDTAVPTFVAVQIAVWLWFTVLFANFAEALAEGRGKARADSLKAGSEGLSARHKE
ADGSFKVVPATSLRKGDVVRVAAGEMIPGDGEVIEGIAAVNEAAITGESAPVIRESGGDR
SAVTGNTRLVSDWLLIRITANPGESTLDRMIALVEGAKRQKTPNEVALDILLIGLTLIFL
LVVVTLQPFAHFANGSLPLVFLVALLVTLIPTTIGGLLSAIGIAGMDRLVRLNVIAKSGR
AVEAAGDVHVLLLDKTGTITFGNRRCAAVVAAPGVSGKEVAEGALFASLADDTAEGKSIV
EYLRALHPQAEPTADELTAVPFSAETRLSGVDYQGRVFRKGAVDSLLAFIGLQRRDLQPA
LSREIDKIAQSGGTPLLVCADGKLLGAIHLKDVVKPGIRERFAELRKLGIRTVMVTGDNP
LTAAAIAAEAGVDDVLAEATPEKKLARIRHEQNDGRLVAMCGDGANDAPALAQADVGMAM
NDGTQAAREAANMVDLDSDPTKLLDVVQIGKELLVTRGALTTFSIANDVAKYFAILPALF
ASIYPQLGVLNVMHLQSPQSAILSAIVFNALIIVVLIPLALRGVRVQAASAAALLRRNLL
IYGLGGILVPFVGIKAIDMLLTALHLV