Protein Info for PFLU_RS08145 in Pseudomonas fluorescens SBW25-INTG

Annotation: NAD-dependent epimerase/dehydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 7 to 126 (120 residues), 31.7 bits, E=4.5e-11
• PF02719: Polysacc_synt_2
  • amino acids 7 to 282 (276 residues), 358.5 bits, E=1.3e-110
• PF16363: GDP_Man_Dehyd
  • amino acids 8 to 236 (229 residues), 62.3 bits, E=2.9e-20
• PF05368: NmrA
  • amino acids 8 to 123 (116 residues), 22.9 bits, E=2.6e-08
• PF01370: Epimerase
  • amino acids 8 to 219 (212 residues), 89.7 bits, E=1e-28
• PF07993: NAD_binding_4
  • amino acids 9 to 142 (134 residues), 28.8 bits, E=3.5e-10
• PF01073: 3Beta_HSD
  • amino acids 9 to 130 (122 residues), 69.8 bits, E=1.1e-22
• PF13460: NAD_binding_10
  • amino acids 11 to 149 (139 residues), 46.3 bits, E=2.3e-15
• PF08485: Polysacc_syn_2C
  • amino acids 285 to 332 (48 residues), 76 bits, 7.3e-25

Best Hits

Swiss-Prot: 63% identical to CAPD_RICFE: UDP-glucose 4-epimerase (capD) from Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1657)

MetaCyc: 80% identical to UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (Pseudomonas aeruginosa O11)
UDP-N-acetylglucosamine 4,6-dehydratase (inverting). [EC: 4.2.1.115]

Predicted SEED Role

"UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-)" in subsystem N-linked Glycosylation in Bacteria (EC 4.2.1.-)

MetaCyc Pathways

• UDP-N-acetyl-β-L-fucosamine biosynthesis (2/4 steps found)
• UDP-N-acetyl-β-L-quinovosamine biosynthesis (1/4 steps found)
• CMP-pseudaminate biosynthesis (1/6 steps found)
• CMP-diacetamido-8-epilegionaminic acid biosynthesis (1/7 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (9/24 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Fatty acid biosynthesis
• Limonene and pinene degradation
• Nucleotide sugars metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Porphyrin and chlorophyll metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.-

Use Curated BLAST to search for 4.2.1.- or 4.2.1.115

Sequence Analysis Tools

See C3K722 at UniProt or InterPro

Protein Sequence (344 amino acids)

>PFLU_RS08145 NAD-dependent epimerase/dehydratase family protein (Pseudomonas fluorescens SBW25-INTG)
MFKSKVLMITGGTGSFGNAVLKRFLETDIAEIRIFSRDEKKQDDMRKHYNNKKLKFYIGD
VRDYQSVLNASRGVDYIFHAAALKQVPSCEFHPMEAVKTNIVGTENLLEAAIQSDVKRVV
CLSTDKAVYPINAMGISKAMMEKVVVAKSRNVDESKIIICGTRYGNVMASRGSVIPLFIE
QIRAGKPLSITDPKMTRFMMTLSDAVDLVLYAFEHANNGDFFVQKAPGATVETLARSLTA
LMGKADHPIKIIGTRHGEKLYEALLSREEMACAEDMGNYFRIPPDLRDLNYSKFVEEGEE
KISKMDDYNSHNTTRLDSDGMQELLMKLEFMRAIKRGEYVTPEE