Protein Info for PFLU_RS06900 in Pseudomonas fluorescens SBW25-INTG

Annotation: beta-glucosidase BglX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 763 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 42 to 345 (304 residues), 259.1 bits, see alignment E=1e-80 PF01915: Glyco_hydro_3_C" amino acids 390 to 646 (257 residues), 211.6 bits, see alignment E=1.7e-66 PF14310: Fn3-like" amino acids 684 to 752 (69 residues), 77.2 bits, see alignment E=1.3e-25

Best Hits

Swiss-Prot: 64% identical to BGLX_SALTY: Periplasmic beta-glucosidase (bglX) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to pfs:PFLU1399)

MetaCyc: 63% identical to beta-D-glucoside glucohydrolase, periplasmic (Escherichia coli K-12 substr. MG1655)
Beta-glucosidase. [EC: 3.2.1.21]

Predicted SEED Role

"Periplasmic beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KC09 at UniProt or InterPro

Protein Sequence (763 amino acids)

>PFLU_RS06900 beta-glucosidase BglX (Pseudomonas fluorescens SBW25-INTG)
MKKLCLLGLVATLATHPVWAETKPAALKDKDAFVSDLLKQMTLDEKIGQLRLISIGPEMP
RELIRKEIAAGRIGGTFNSITRPENRPMQDAAMRSRLKIPMFFAYDVIHGHRTIFPISLA
LASSWDMEAIGRSGRIAAQEAAADSLDITFAPMVDISRDPRWGRTSEGFGEDTYLVSRIA
EVMVKAFQGVSPANADSIMASVKHFALYGAVEGGRDYNVVDMSPVKMYQDYLPPYHAAIK
AGSGGVMVALNSINGVPATANTWLMNDLLRKDWGFKGLAVSDHGAIFELIKHGVAKDGRE
AAKLAIKAGIDMSMNDSLYGKELPGLLKSGEIEQSDIDNAVREVLGAKYDMGLFKDPYLR
IGKAEDDPADTYAESRLHRADARDIARRSLVLLKNQNNTLPLKKSATIALVGPLAKAPID
MMGSWAAAGKPEQSVTLLDGLNAVIGEKGKILYARGANITNDKAVVDYLNFLNFDAPEVV
DDTRPAQVMIDEAVKAAKEADVVVAAVGESRGMSHESSSRTDLNLPQSQRDLIKALKATG
KPLVLVLMNGRPLSILEENQQADAILETWFAGTEGGNAIADVLFGDYNPSGKLPITFPRS
VGQIPTYYNHLTIGRPFTPGKPGNYTSQYFDDTTGPLFPFGYGLSYTTFGLSDMALSSTT
LNKTGKLDASVTVTNTGKVDGETVVQLYIQDVAGSMIRPIKELKNFQKVMLKAGEERTLH
FTITEEDLKFYNTQLKYAAEPGEFNVQIGLDSQDVQQQTFELL