Protein Info for PFLU_RS06805 in Pseudomonas fluorescens SBW25-INTG

Annotation: efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1053 transmembrane" amino acids 10 to 28 (19 residues), see Phobius details amino acids 339 to 359 (21 residues), see Phobius details amino acids 366 to 389 (24 residues), see Phobius details amino acids 395 to 415 (21 residues), see Phobius details amino acids 438 to 459 (22 residues), see Phobius details amino acids 471 to 495 (25 residues), see Phobius details amino acids 539 to 559 (21 residues), see Phobius details amino acids 879 to 897 (19 residues), see Phobius details amino acids 904 to 924 (21 residues), see Phobius details amino acids 930 to 953 (24 residues), see Phobius details amino acids 979 to 999 (21 residues), see Phobius details amino acids 1010 to 1036 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1033 (1033 residues), 1328 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1050 (1050 residues), 1796.6 bits, see alignment E=0

Best Hits

Swiss-Prot: 81% identical to MEPB_PSEPU: Multidrug/solvent efflux pump membrane transporter MepB (mepB) from Pseudomonas putida

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 63% identity to abo:ABO_0964)

MetaCyc: 65% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBN3 at UniProt or InterPro

Protein Sequence (1053 amino acids)

>PFLU_RS06805 efflux RND transporter permease subunit (Pseudomonas fluorescens SBW25-INTG)
MSKFFIDRPIFAWVIALVIMLVGALSILKLPINQYPAIAPTAIDIQVTYPGASAQTVQDT
VVQVIEQQLNGIDNLRYVASDSNSDGSMTITATFNQGTNPDIAQVQVQNKLNLATPLLPQ
EVQQQGIRVTKSVKNFLMVIGLVSEDGSMTKDDLSNYIVSNIQDPISRTAGVGDFQVFGS
QYAMRIWLDPAKLNNFQLTPVDVSTAISAQNVQVATGQLGGLPALPGNQLNATIIGKTRL
QTAEQFGNILMKVNADGSQVRLHDIARIELGGQNYSISAQFNGNPASGMAIKLASGANAL
DTAKAIRATVASLEPFFPPGMKAVVPYDTTPVVTESISGVVHTLVEAIVLVFLVMFLFLQ
NFRATIITTMTVPVVLLGTFGILAAFGFTINTLTMFGMILAIGLLVDDAIVVVENVERVM
AEEHLSPKEATVKSMGQIQGALVGIALVLSAVLLPMAFFGGSTGVIYKQFSITIVSAMAL
SVLVALIFTPALCATMLKPIDPEKHGQPKRGFFGWFNRTFDRGVLSYERGVGNMIKHKIP
AFLVYALIFAGMIWLFMRIPAAFLPDEDQGVIFAQVQTPVGSSAERTQKVIDDMRSYLLN
DKEGEPGEGKGVKSVFTVNGFNFAGRGQSSGLAFVMLKPWDERDASTSVFEIAKRAQGYF
MQTFQDAMVFAIVPPSVLELGNATGFDVFLQDQGGVGHEKLMAARNQFLGMAAQSKILAG
VRPNGVNDEPQYELTVDDEKASAQGITLSNINQTLAIALGGSYVNDFIDRGRVKKVYVQG
EAASRMSPEDLNKWYVRSDSGKMVPLSAIASGKWIFGSPKLSRYNGVAAMEILGTPAPGY
STGDAMAEVERIAKQLPAGIGYAWTGLSYEERLSGSQAPALYALSLLVVFLCLAALYESW
SIPIAVVLVVPLGVVGALIATSMRGLSNDVFFQVGLLVTVGLAAKNAILIVEFAKELHEQ
GKGIVEAAIEASRMRLRPIIMTSMAFILGVLPLAISSGAGSGSQHAIGTGVIGGMITATV
LAIFWVPLFFATVSAIGDRKPKETKQTPKEAGQ