Protein Info for PFLU_RS06495 in Pseudomonas fluorescens SBW25-INTG

Annotation: penicillin acylase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 813 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF01804: Penicil_amidase" amino acids 52 to 799 (748 residues), 605.8 bits, see alignment E=8.7e-186

Best Hits

Swiss-Prot: 79% identical to QUIP_PSEPF: Acyl-homoserine lactone acylase QuiP (quiP) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01434, penicillin amidase [EC: 3.5.1.11] (inferred from 100% identity to pfs:PFLU1314)

Predicted SEED Role

"Penicillin acylase (EC 3.5.1.11)" (EC 3.5.1.11)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.11

Use Curated BLAST to search for 3.5.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7I9 at UniProt or InterPro

Protein Sequence (813 amino acids)

>PFLU_RS06495 penicillin acylase family protein (Pseudomonas fluorescens SBW25-INTG)
MASPALSHFLPRFGVAAAVASALSLAGCQLQSTQDTLPPVAGVQPIKGLAQNVSVRRNAQ
GMPLIESNTFHDALFSLGYVHASDRITQMVTLRLLAQGRLAEMSGPDVLDVDRFMRAVNL
KKSAGELYNASSPRLKRFFEVYARGVNAYLFRYRDKLPPDLAQTGYKPEYWKPEDSALLF
CLLNFSESANLQEEISSLVLAQKVGVDKLAWLTPSAPDEAIPLAEAEKLKGVNLGQVTGL
AGLDAVGQQLSSLNSLAVTTSSNWAIGPQRSRSGKSLLAGDLAAQPQAPSPWSYVQIRAP
KYQAVGASIAGLPTLLSGFNGKVAWSMSTVKGDTQDLFLEKVRRQGSALYYENNGKWLPA
GVRNETFFVKGQRSIREVVYETRHGALLNSSQALTTGLGLALQTADFKDDKSLDAFFDLS
RAQNAGKASDATREIRAIALNMVFADASNIGWQVTGRFPNRREGEGLLPSPGWDTRFDWD
GYADAMLHPYDQDPPQGWIGTANQRTAPRGYGMQLSNSWDAPERSERLAQLANAGKHDSR
SMIAMQYDQTTTFAAKLKTMFQAPGMAQPLKQAIDALPAAEQAKAREALSRLMAFDGRLV
ATSADAALYELFLQESTKQIFLDELGPENSASWKAFVSNASLSYAAQADHLLGREDSPFW
DDVRTPQKEDKPAILARSLAAAISVGDSQLGADHKAWQWGKLHTTTWKNINGQVIRGPLA
SGGDHNTLNPAPYSWGQDFTTTQVPALRMIVDFGQAEPMMGQGGIGQSGNPASPNYATGI
DPSLKAQYLSFPMQPQNFEKVYGKARLTLTPGK