Protein Info for PFLU_RS06265 in Pseudomonas fluorescens SBW25-INTG

Annotation: [protein-PII] uridylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 PF03445: DUF294" amino acids 34 to 128 (95 residues), 22.2 bits, see alignment E=2.7e-08 TIGR01693: protein-P-II uridylyltransferase" amino acids 36 to 885 (850 residues), 1095 bits, see alignment E=0 PF01909: NTP_transf_2" amino acids 65 to 116 (52 residues), 33.7 bits, see alignment 9.7e-12 PF08335: GlnD_UR_UTase" amino acids 185 to 322 (138 residues), 154.8 bits, see alignment E=3.6e-49 PF01966: HD" amino acids 487 to 563 (77 residues), 38.9 bits, see alignment E=2.5e-13 PF01842: ACT" amino acids 815 to 866 (52 residues), 32.7 bits, see alignment 1.3e-11

Best Hits

Swiss-Prot: 100% identical to GLND_PSEFS: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 100% identity to pfs:PFLU1268)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5E4 at UniProt or InterPro

Protein Sequence (900 amino acids)

>PFLU_RS06265 [protein-PII] uridylyltransferase (Pseudomonas fluorescens SBW25-INTG)
MPQVDPELFDRGQFQAELALKASPIAAFKKAIRQAREVLDGRFRSGRDIRRLIEDRAWFV
DNILQKAWEQFSWSEDADIALVAVGGYGRGELHPYSDIDLLILLDSADHEIFRDSIERFL
TLLWDIGLEVGQSVRSVDECAEEARADLTVITNLMESRTIAGPERLRQRMLEVTSTAHMW
PSKDFFLAKRAEQKARHHKYNDTEYNLEPNVKGSPGGLRDIQTILWVARRQYGTLNLRAL
AGEGFLVESENALLASSQEFLWKVRYALHMLAGRSEDRLLFDHQRSIATLLGFEGEDAKT
SIESFMQQYYRVVMSIAQLSDLIIQHFEEVILAPEDEAPPQPINSRFQLHDGYIEARNDN
VFRRTPFAMLEIFVLMAQQPEIKGVRADTIRLLRENRHLIDDDFRNDIRNTSLFIELFKC
KIGIHRNLRRMNRYGILGRYLPEFGFIVGQMQHDLFHIYTVDAHTLNLIKHLRKLQYTQV
SEKFPLASKLMAKLPKPELIYLAGLYHDIGKGRHGDHSEIGAVDAEAFCQRHQLPLWDSR
LIVWLVQNHLVMSTTAQRKDLSDPQVIHDFAGIVEDETRLDYLYVLTVSDINATNPTLWN
SWRASLLRQLYTETKRALRRGLENPVDREEQIRRTQSAALDILVRGGNDPDDVEQLWSQL
GDDYFLRHTAGDVAWHSDAILQQPADGGPLVLIKETTQREFEGGTQIFIYAPDQHDFFAV
TVAAMDQLNLNIHDARVITSSSQFTLDTYIVLDTEGESIGDNPVRVKKIREGLTEALRNP
DDYPTIIQRRVPRQLKHFAFAPQVTISNDAQRPVTVLELSAPDRPGLLARIGGIFLEFDL
SLQNAKIATLGERVEDVFFITDADNQPLSDPELCRRLQDAIVQQLSVTQEPGVELTRLTI