Protein Info for PFLU_RS05625 in Pseudomonas fluorescens SBW25-INTG

Annotation: sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 633 transmembrane" amino acids 25 to 45 (21 residues), see Phobius details amino acids 317 to 338 (22 residues), see Phobius details PF02743: dCache_1" amino acids 52 to 302 (251 residues), 73.3 bits, see alignment E=3.3e-24 PF00512: HisKA" amino acids 411 to 475 (65 residues), 30.2 bits, see alignment E=5.7e-11 PF02518: HATPase_c" amino acids 517 to 625 (109 residues), 81.7 bits, see alignment E=7.8e-27

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 100% identity to pfs:PFLU1135)

Predicted SEED Role

"Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3)" (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAM3 at UniProt or InterPro

Protein Sequence (633 amino acids)

>PFLU_RS05625 sensor histidine kinase (Pseudomonas fluorescens SBW25-INTG)
MKCDPNQFRAAPPSLAVKPRLIRQLFLPPLIVALMIGLGYIGFWISEYYGIRTLSDTGER
QLELHARTVESELSKYTYLPSLLELESSVSTLLADPNQETRKTVNDYLEGLNRRSRSRAI
YVMDTTGRVLATSNWRDADSYQGEDLSFRAYFQNAVRGQPGRFYGIGSTNGEPGYYLAHG
LEEHGKIIGVAVVKVRLEALEERWQRARLEAFVSDENGIIILSSDPARRLKAVRPLSDDT
KERLAHSLQYYWATLNELEPLARERLNEVSEKLTFPANSEVVNDEHEVSYLAQTRPLNDT
PWNFTLLTPLNDLRRAAINQGILVAVAFGLVAFLLIAWNERRKVIATRLAAREALQEANS
QLERRIAERTADLRASNERLKGQIRERRQAEETLRRAQDELVQAGKLAAIGQMSTSIAHE
LNQPLAALRTLSGNTVRFLERGALDTASANLKTINELIDRMGRITASLRSFARRGDDQGE
ASLAKAVDAAFQVLGARLDNLPLTLHRDFSNAQLQIDQTRLEQILVNLIGNALDAMQAQP
APELWLEGSTVDGKYRLQVRDNGHGIDPEARKHLFEPFFTTKPGEQGLGLGLTLSASLAA
ATGGNLAVEHPASGGTAFVLCLPLAGAQKAEST