Protein Info for PFLU_RS05270 in Pseudomonas fluorescens SBW25-INTG

Annotation: DNA repair protein RecO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 PF11967: RecO_N" amino acids 11 to 75 (65 residues), 43 bits, see alignment E=3.6e-15 TIGR00613: DNA repair protein RecO" amino acids 13 to 148 (136 residues), 62.5 bits, see alignment E=2e-21 PF02565: RecO_C" amino acids 83 to 224 (142 residues), 69.7 bits, see alignment E=2.7e-23

Best Hits

Swiss-Prot: 90% identical to RECO_PSEPF: DNA repair protein RecO (recO) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03584, DNA repair protein RecO (recombination protein O) (inferred from 100% identity to pfs:PFLU1062)

MetaCyc: 40% identical to recombination mediator protein RecO (Escherichia coli K-12 substr. MG1655)
RXN0-2606

Predicted SEED Role

"DNA recombination and repair protein RecO" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6Y9 at UniProt or InterPro

Protein Sequence (229 amino acids)

>PFLU_RS05270 DNA repair protein RecO (Pseudomonas fluorescens SBW25-INTG)
MSQSPPPSQLAYVLHSRAYRETSALVDFLTPQGRLRAVLRSARGKAGTLARPFVALDVEF
RGKGELKNVGRLESVGTSAWLNGEALFSGLYLNELLIRLLPAEDPHPAVFDHYAATLIAL
AEGRPLEPLLRAFEWRLLDDLGYGFELNNDLHGDPIAADGMYRLQVDAGLERVYLLQPGL
FQGTELLAMSEADWTAPGALSAAKRLMRQALAVHLGGRPLVSRELFRKP