Protein Info for PFLU_RS05215 in Pseudomonas fluorescens SBW25-INTG

Annotation: 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 PF05971: Methyltransf_10" amino acids 28 to 324 (297 residues), 428.8 bits, see alignment E=1.2e-132 PF05175: MTS" amino acids 132 to 220 (89 residues), 25 bits, see alignment E=1.3e-09

Best Hits

Swiss-Prot: 100% identical to RLMF_PSEFS: Ribosomal RNA large subunit methyltransferase F (rlmF) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 100% identity to pfs:PFLU1051)

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6G1 at UniProt or InterPro

Protein Sequence (339 amino acids)

>PFLU_RS05215 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF (Pseudomonas fluorescens SBW25-INTG)
MTAPSTPKPQRKKPKTATTAKPVVPRKEATLHPRNRHTGRYDFQALIKTTPELAQFVIIN
PYGKESIDFASPDAVRVFNRALLKSFYGIQHWDIPADYLCPPVPGRADYVHFLADLLASV
NDGKIPRGSIVKVLDIGMGANCVYPLIGYMEYRWNFLGSEVDPIAVAAAKAIVQSNDLSK
VIQLRQQTNPKQILLSLLEPGERFDLTMCNPPFHASMDEATKGSERKWRALGKADPKRKL
PVLNFGGQSAELWCEGGEARFVTQLIAESAHFAHKVLWFSTLVSKASNLPAIETALKKAG
VLESQVVEMSQGQKQSRFVAWTFQTKNEQQIWRQRWVRD