Protein Info for PFLU_RS04910 in Pseudomonas fluorescens SBW25-INTG

Annotation: glycosyltransferase family 2 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 44 to 73 (30 residues), see Phobius details amino acids 374 to 392 (19 residues), see Phobius details amino acids 397 to 418 (22 residues), see Phobius details amino acids 429 to 447 (19 residues), see Phobius details amino acids 475 to 495 (21 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 89 to 345 (257 residues), 136.7 bits, see alignment E=1.1e-43 PF13632: Glyco_trans_2_3" amino acids 186 to 403 (218 residues), 93.4 bits, see alignment E=1.8e-30

Best Hits

Swiss-Prot: 90% identical to ALG8_PSESM: Glycosyltransferase alg8 (alg8) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU0989)

MetaCyc: 82% identical to mannuronosyl transferase catalytic subunit (Pseudomonas aeruginosa)
Alginate synthase. [EC: 2.4.1.33]

Predicted SEED Role

"Alginate biosynthesis protein Alg8" in subsystem Alginate metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDZ9 at UniProt or InterPro

Protein Sequence (496 amino acids)

>PFLU_RS04910 glycosyltransferase family 2 protein (Pseudomonas fluorescens SBW25-INTG)
MTLMSKLKHVLLQSAGWLFFLSLLMGLAALLPASTFDSESKNFIFLIGAVGIWRYSMGAT
HFFRGMLFLYVVYPHLRRKVRKLGKAADPSHVFLMVTSFRIDALTTAQVYSSVIREAIEC
GFPTTVVCSLVEMSDELLVKSLWERMNPPEHVKLDFVRIAGTGKRDGLAFGFRAISRHLP
DDRAVVAVIDGDTVLAEGVVRKTVPWFQLFGNVGGLTTNEFCEVRGGYIMSEWHKLRFAQ
RHINMCSMALSKRVLTMTGRMSVFRASVVTDPGFIADVESDSLQHWRLGRFKFLTGDDKS
SWFSLMRLGYDTFYVPDAAINTVEHPPEKSFIKASRKLMFRWYGNNLRQNSRALGLGVRR
LGLFTSVVLFDQRVSMWTSLLGLTVAIIATFKYGGAFILAYLLWIGITRLILTLLLSCSG
HTIGPAYPMILYYNQIMGALVKIYVFFRLDQQSWTRQDTKLTRDLASFQRWFNTWSSRTM
TFSAGSIFVAVLLMMV