Protein Info for PFLU_RS04890 in Pseudomonas fluorescens SBW25-INTG

Annotation: right-handed parallel beta-helix repeat-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF13229: Beta_helix" amino acids 274 to 419 (146 residues), 54.5 bits, see alignment E=1.1e-18 PF05048: NosD" amino acids 274 to 368 (95 residues), 29.7 bits, see alignment E=4.4e-11 amino acids 363 to 429 (67 residues), 35.3 bits, see alignment E=8.7e-13 TIGR03804: parallel beta-helix repeat" amino acids 363 to 401 (39 residues), 22.4 bits, see alignment 3.8e-09 amino acids 381 to 419 (39 residues), 23.8 bits, see alignment 1.3e-09

Best Hits

Swiss-Prot: 100% identical to ALGG_PSEFL: Mannuronan C5-epimerase (algG) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU0985)

MetaCyc: 69% identical to mannuronan C5 epimerase (Pseudomonas aeruginosa)
RXN-9839 [EC: 5.1.3.37]; TRANS-RXN-269 [EC: 5.1.3.37]

Predicted SEED Role

"Poly (beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-)" (EC 5.1.3.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.- or 5.1.3.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDZ5 at UniProt or InterPro

Protein Sequence (529 amino acids)

>PFLU_RS04890 right-handed parallel beta-helix repeat-containing protein (Pseudomonas fluorescens SBW25-INTG)
MGACAMNPQALKGSAMLAAAMLLASGAAMADVAPQAKAPTIAKELQQAKTYTISSPPTAP
LEMAKPALPDLSGYTDAAMEKKIVRAKPGKISIRRMMQEDALKDFIGGDNKMAEWVVRQH
GIPQAIFIDDGYMNLKDLLGKVPKQYLSETSPGVFLAKLPIVVGRKGILEIDKKTQELRL
SQEAGSFLINDGQLFVRDTKVTGWSEKANGPALYKSPKEFRPFLLAWGGTETYISNTKMA
SFGYANSKSYGVSISQYTPNMAKVLKRPEPTGWIIDSEFSDMWYGFYCYETTGFVIKGNT
YKDNIVYGIDPHDRSHGLIIADNTVYGTKKKHGIIISREVNDSFIFNNRSYDNKLSGLVL
DRNSVNNFVADNEFYRNHTDGITLYESGDNLLWGNKVIANRRHGIRVRNSVNIKLYENTS
MANGLTGLYGHIKDLTDTDRDIALDPFDAKVSLIVVGGELAGNGSGPLSIDSPLSVELYR
VSMLAPTKSSGISFNGVLGDRQEEILDLLVRQQKAVLIDPVERQTELQD