Protein Info for PFLU_RS04560 in Pseudomonas fluorescens SBW25-INTG

Annotation: M48 family metalloprotease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01435: Peptidase_M48" amino acids 73 to 257 (185 residues), 130.5 bits, see alignment E=3.3e-42

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU0917)

Predicted SEED Role

"Zn-dependent protease with chaperone function"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBK6 at UniProt or InterPro

Protein Sequence (272 amino acids)

>PFLU_RS04560 M48 family metalloprotease (Pseudomonas fluorescens SBW25-INTG)
MKKSMAISGLVAAMLLAGCQSVNTTSGGAVGVERKQYMFSMLSSQEVDQMYAQSYQQTLG
EASSKGQLDKTSANAKRVQAIAKRLIAQAPTFRPDAAQWKWEVNLIKSDEMNANCGPGGK
IFVYSALIDNLKLTDDELAAVMGHEIAHALREHGREAMSKAYGIEMAKQGAGALFGLGQD
SLALADTVANYGMTLPNSRSNENEADLIGLELSARAGYNPNAAITLWNKMAKASEGSPPE
FMSTHPASDSRIASLQAAIPKVMPLYQQAKKS