Protein Info for PFLU_RS04470 in Pseudomonas fluorescens SBW25-INTG

Annotation: histidinol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 PF00815: Histidinol_dh" amino acids 30 to 433 (404 residues), 573.5 bits, see alignment E=1.4e-176 TIGR00069: histidinol dehydrogenase" amino acids 39 to 433 (395 residues), 541.1 bits, see alignment E=9.4e-167

Best Hits

Swiss-Prot: 93% identical to HISX_PSEPF: Histidinol dehydrogenase (hisD) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00013, histidinol dehydrogenase [EC: 1.1.1.23] (inferred from 100% identity to pfs:PFLU0896)

Predicted SEED Role

"Histidinol dehydrogenase (EC 1.1.1.23)" in subsystem Histidine Biosynthesis (EC 1.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.23

Use Curated BLAST to search for 1.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K8X6 at UniProt or InterPro

Protein Sequence (440 amino acids)

>PFLU_RS04470 histidinol dehydrogenase (Pseudomonas fluorescens SBW25-INTG)
MTTSTAIARLNAADPDFAHHLDHLLSWESVSDDSVNQRVLDIIKAVRERGDAALVDFTRQ
FDGLDVKSMSDLILPRERLELALTRITAPQREALEVAASRVRSYHEKQKQDSWSYTEADG
TVLGQKVTPLDRAGLYVPGGKASYPSSVLMNAIPAKVAGVTEVVMVVPTPRGEINELVLA
AACIAGVDRVFTIGGAQAVAALAYGTESVPKVDKVVGPGNIYVATAKRHVFGQVGIDMIA
GPSEILVVCDGQTDPDWIAMDLFSQAEHDEDAQAILVSPDAEFLDQVAASINKLLPTMER
AEIIEKSINGRGALILVRDMDQAIEVANRIAPEHLELSVADPQAWLPSIRHAGAIFMGRH
TSEALGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRSSIIFCSPQGASELGKTASVLARG
ESLSAHARSAEYRILEEKGN