Protein Info for PFLU_RS04120 in Pseudomonas fluorescens SBW25-INTG

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00496: SBP_bac_5" amino acids 71 to 457 (387 residues), 336 bits, see alignment E=1.6e-104

Best Hits

Swiss-Prot: 51% identical to DPPA_ECOLI: Periplasmic dipeptide transport protein (dppA) from Escherichia coli (strain K12)

KEGG orthology group: K12368, dipeptide transport system substrate-binding protein (inferred from 100% identity to pfs:PFLU0826)

MetaCyc: 51% identical to dipeptide ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-8-RXN [EC: 7.4.2.9]

Predicted SEED Role

"Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2)" in subsystem ABC transporter dipeptide (TC 3.A.1.5.2) or Bacterial Chemotaxis (TC 3.A.1.5.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5B9 at UniProt or InterPro

Protein Sequence (542 amino acids)

>PFLU_RS04120 ABC transporter substrate-binding protein (Pseudomonas fluorescens SBW25-INTG)
MLKHAVLPLLVSAGLMAAAPVALAATNLVFCSEGSPAGFDPGQYTTGTDFDASAETMFNR
LTQFERGGTAVIPGLATSWDVSPDGLTYTFHLRDGVKFHTTAYFKPTRTFNADDVLFTFN
RMINKDDPFRKAYPTEFPYFTDMGMDTNIKNIEKVDEHTVKFTLGTVDAAFIQNLAMSFA
SIQSAEYAAQLLKEGKASDINQKPVGTGPFVFKSYQKDSNIRYTGNKDYWKPEDVKIDNL
IFAITTDPSVRIQKLKKNECQITLFPRPADLKALEADKDLKLPNQAGFNLGYIAYNVMDK
VKGSDQPNPLADLRVRQALDMSVNKQQIIDSVYQGAGQLAVNAMPPTQWSYDTTIKDAKY
DPEKAKTLLKEAGVKEGTEIVLWAMPVQRPYNPNAKLMAEMLQNDWKKIGLNVKITSYEW
GEYIKRSKGGENQAMIIGWSGDNGDPDNWLNVLFGCDSLSGNNFSKWCDKKFDGIVKEAK
ATSDVAKRTELYKQAQHILKDAVPMTPIAHSTVYQPMRTTVQDFKISPFGLNSFYGVSVS
GK