Protein Info for PFLU_RS03855 in Pseudomonas fluorescens SBW25-INTG

Annotation: glycine oxidase ThiO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00890: FAD_binding_2" amino acids 7 to 211 (205 residues), 40.1 bits, see alignment E=5.5e-14 PF01266: DAO" amino acids 7 to 347 (341 residues), 247 bits, see alignment E=8.6e-77 TIGR02352: glycine oxidase ThiO" amino acids 7 to 349 (343 residues), 274.2 bits, see alignment E=8.2e-86

Best Hits

Swiss-Prot: 81% identical to GLYOX_PSEPK: Glycine oxidase (thiO) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03153, glycine oxidase [EC: 1.4.3.19] (inferred from 100% identity to pfs:PFLU0777)

Predicted SEED Role

"Glycine oxidase ThiO (EC 1.4.3.19)" in subsystem Thiamin biosynthesis (EC 1.4.3.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDY3 at UniProt or InterPro

Protein Sequence (369 amino acids)

>PFLU_RS03855 glycine oxidase ThiO (Pseudomonas fluorescens SBW25-INTG)
MAKQQQVVIVGGGVIGLLTAFNLATQGQAVVLLERAGLGQESSWAGGGIVSPLYPWRYSP
AVTALAHWSQDFYPQLAEHLFAATGVDPEVHTTGLYWLDLDDEAEALAWAAREGRPLSKV
DISAAHDAVPVLGGGYSQAIYMANVANVRNPRLVKSLKAALLALPGVTLHEQCEVTGFVV
DAGSVVGVNTAQGPVLGDQVVLAAGAWSGELLGTLGLSLPVEPVKGQMILYKCASDFLSS
MVLAKGRYAIPRRDGHILIGSTLEHEGFDKTPTDKALESLKASAVELIPALADAEVVGHW
AGLRPGSPEGIPYIGPVPGFKGLWLNCGHYRNGLVLAPASCQLFADLLLMRAPIIDPAPY
APEGRINAG