Protein Info for PFLU_RS03735 in Pseudomonas fluorescens SBW25-INTG

Annotation: NAD(P)/FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF13450: NAD_binding_8" amino acids 7 to 61 (55 residues), 50.2 bits, see alignment E=5.3e-17 PF01593: Amino_oxidase" amino acids 12 to 69 (58 residues), 37.7 bits, see alignment E=3.3e-13 amino acids 108 to 324 (217 residues), 60.1 bits, see alignment E=5.3e-20

Best Hits

Swiss-Prot: 81% identical to RNLS_PSE14: Renalase (PSPPH_1014) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K06955, (no description) (inferred from 100% identity to pfs:PFLU0754)

Predicted SEED Role

"COG3380: Amine oxidase, flavin-containing"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDN5 at UniProt or InterPro

Protein Sequence (328 amino acids)

>PFLU_RS03735 NAD(P)/FAD-dependent oxidoreductase (Pseudomonas fluorescens SBW25-INTG)
MTVPIAIIGTGIAGLSAARALRDAGHVVQLFDKSRGSGGRMSSKRSDAGSLDMGAQYFTA
RDRRFVNEVQRWQSNGWAEQWKPQLYNFKSGQLTPSPDEQIRWVGTPRMSAITRALLDDL
PVEFGCRITEVFQGKQHWNLLDADSENHGPFSHVIIATPAPQATALLAAAPKLASAAAGV
KMDPTWAIALAFDKPLDTPMEGCFVQDSPLDWLARNRSKPGRDTALDTWVLHATSAWSKA
HLDLSKEAVIEHLHGAFAELLHSAMPAPSFSLAHRWLYARPSSSHEFGVLADADLGLYVC
GDWCLSGRVEGAWLSGQEAARRLIEHLQ