Protein Info for PFLU_RS03605 in Pseudomonas fluorescens SBW25-INTG

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 PF14532: Sigma54_activ_2" amino acids 21 to 181 (161 residues), 59.8 bits, see alignment E=9.2e-20 PF00158: Sigma54_activat" amino acids 25 to 177 (153 residues), 196.7 bits, see alignment E=6e-62 PF07728: AAA_5" amino acids 34 to 150 (117 residues), 29.8 bits, see alignment E=1.4e-10 PF02954: HTH_8" amino acids 263 to 302 (40 residues), 29.7 bits, see alignment 1.1e-10

Best Hits

Swiss-Prot: 60% identical to WTSA_PANSE: Pathogenicity locus probable regulatory protein WtsA (wtsA) from Pantoea stewartii subsp. stewartii

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU0728)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KCR7 at UniProt or InterPro

Protein Sequence (305 amino acids)

>PFLU_RS03605 sigma-54-dependent Fis family transcriptional regulator (Pseudomonas fluorescens SBW25-INTG)
MSASDTMDNEADISLLEGPDIEEILSGTAQLNIDILLLGETGTGKDTLAQRIHRLSGRRG
SFIAVNCAAIPETLAESQLFGVNSGAYTGAVQSRAGFIEAAHLGTLYLDEIDSMPLSLQA
KLLRTLESRGVERLGSTRFIPVDMRVIASAQQSLYEMVERGTFRRDLYFRLSVVNIQLPS
LRECRDRIIPLFLTMIRQEAESFKCPYPQPPSSLLQQLLCHPWPGNVRELSSTAKRFVLG
LPPLSVRGKNPKISEASLKERLQRIEKSLIEESLHRHNGNVDLAAADLRVAKRTFYYRMK
QLGID