Protein Info for PFLU_RS03495 in Pseudomonas fluorescens SBW25-INTG

Annotation: methyl-accepting chemotaxis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 PF08448: PAS_4" amino acids 31 to 135 (105 residues), 42.9 bits, see alignment E=1.3e-14 amino acids 154 to 258 (105 residues), 35.6 bits, see alignment E=2.3e-12 TIGR00229: PAS domain S-box protein" amino acids 33 to 141 (109 residues), 33.8 bits, see alignment E=1.7e-12 amino acids 141 to 263 (123 residues), 50.7 bits, see alignment E=9.5e-18 PF13426: PAS_9" amino acids 33 to 133 (101 residues), 47.1 bits, see alignment E=6.3e-16 amino acids 156 to 248 (93 residues), 31.6 bits, see alignment E=4.2e-11 PF00989: PAS" amino acids 34 to 127 (94 residues), 25.7 bits, see alignment E=2.4e-09 amino acids 153 to 244 (92 residues), 24.3 bits, see alignment E=6.8e-09 PF08447: PAS_3" amino acids 45 to 125 (81 residues), 27.6 bits, see alignment E=7.2e-10 amino acids 165 to 250 (86 residues), 47 bits, see alignment E=6.2e-16 PF00015: MCPsignal" amino acids 276 to 433 (158 residues), 126.7 bits, see alignment E=2.2e-40

Best Hits

Swiss-Prot: 45% identical to BDLA_PSEAE: Biofilm dispersion protein BdlA (bdlA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03406, methyl-accepting chemotaxis protein (inferred from 100% identity to pfs:PFLU0704)

Predicted SEED Role

"Methyl-accepting chemotaxis protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBW6 at UniProt or InterPro

Protein Sequence (438 amino acids)

>PFLU_RS03495 methyl-accepting chemotaxis protein (Pseudomonas fluorescens SBW25-INTG)
MFNTRLKQELSALREELSSLDQVKESLESEMLCLTLDAEGRVEWANANFLQELAYQSGHL
LGRAIEDLVPTHVRQDEYQLRFKNALLRGEHFAGTVRLMRGNGQEAWLRSIVQPVRSSDG
RIKHFSMYSSDLTRTIEASREHENLMNALVRSTAVIEFDLNGHVLTANDRFLGGMGYSLA
QIQGKHHRIFCEPEEYNSTEYQDFWKRLNAGEFVAARFKRVDSHGRVVWLEATYNPVLDA
NDRLYKVVKFATVITDQVNQEQAVAEAASIAYSTSLQTDNSAQRGTTVVTQAVDVMRDLA
RHMQQAGDGIEALNAQSQVIGSIVKTISGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
EVRQLASRTSKATEEIVGVVRQNQDMAREAVALMTDGRLQAEQGLALAAEAGTVIVEIQD
GAQKVVSAVGQFANQLST